Anti-cancer effect of the flavonoids of Astragalus combined with cisplatin on Lewis lung carcinoma-bearing mice
10.16438/j.0513-4870.2019-0539
- VernacularTitle:黄芪总黄酮联合顺铂对Lewis荷瘤小鼠抗癌作用的研究
- Author:
Yan-shuang QI
1
;
Xiao LI
1
;
Xue-mei QIN
1
;
Zhi CHAI
2
;
Zhen-yu LI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. College of Basic Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, China
- Publication Type:Research Article
- Keywords:
total flavonoids of Astragalus membranaceus;
Lewis mice;
lung cancer;
network pharmacology;
metabonomics
- From:
Acta Pharmaceutica Sinica
2020;55(5):930-940
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to analyze the anti-cancer effect and mechanism of action of the flavonoids of Astragalus membranaceus (TFA) when combined with cisplatin on Lewis lung carcinoma-bearing mice. This animal experiment was approved by the Committee of the Ethics of Animal Experiment of Shanxi University (SXULL2018012). Pharmacological indices such as tumor weight, tumor volume growth curves, inhibition rate and organ indices showed that the TFA could reduce toxicity and enhance the efficacy of cisplatin. The target of TFA was predicted by network pharmacology analysis and the result showed that calycosin-7-O-β-D-glucoside might be the main active compound responsible for the anticancer effect of TFA. TRP53 (cellular tumor antigen p53), RAC1 (Ras-related C3 botulinum toxin substrate 1), ERBB2 (receptor tyrosine-protein kinase erbB-2), VEGFA (vascular endothelial growth factor A) and STAT3 (signal transducer and activator of transcription 3) may be associated with TFA in enhancing efficacy and reducing the toxicity of cisplatin. The IL-6 content in serum and expression levels of STAT3 and p53 in tumor tissues suggested that TFA may inhibit tumor growth through the IL-6/STAT3 pathway; UPLC-MS-based serum metabolomic analysis suggested that the metabolic pathways related to lung cancer include sphingolipid metabolism, retinol metabolism, glycerophospholipid metabolism, primary bile acid biosynthesis, and the TFA-regulated corresponding pathway of bile acid biosynthesis. In this study, the anti-cancer effect and mechanism of action of TFA combined with cisplatin on Lewis lung carcinoma-bearing mice was analyzed by the combination of various techniques, which lay a foundation for further development of anticancer drugs.
