Characterization of Echinostoma miyagawai in domestic ducks in Wuhu City
10.16250/j.32.1374.2019271
- VernacularTitle:芜湖地区家鸭体内宫川棘口吸虫鉴定
- Author:
Sheng-Li GU
1
;
Mei-Yuan LUO
1
,
2
;
Wei GUO
1
;
Yang PAN
1
;
Xiao-Dong ZHAN
1
Author Information
1. Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China
2. Co-first author
- Publication Type:Journal Article
- Keywords:
Echinostoma miyagawai;
Domestic duck;
Cox1 gene;
Morphological feature;
Molecular characterization;
Wuhu City
- From:
Chinese Journal of Schistosomiasis Control
2020;32(2):191-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the morphological characteristics of Echinostoma miyagawai in domestic ducks in Wuhu area, and to explore the feasibility of the cytochrome oxidase subunit-1 (Cox1) gene as a molecular marker for the identification of E. miyagawai. Methods E. miyagawai was isolated from free-ranged domestic ducks in Wuhu area, and the parasites were stained and identified. In addition, the mitochondrial Cox1 gene of E. miyagawai was amplified using a PCR assay, and the amplification product was sequenced and aligned with the GenBank database to yield the homology for the identification of parasite species in combination with morphological findings. Intra-species comparison was done based on the Cox1 gene sequence. Results The prevalence of E. miyagawai infection was 16.67% in domestic ducks in Wuhu area, and the adult E. miyagawai was 6.6 to 13.2 mm in length. The size of the E. miyagawai Cox1 gene was approximately 660 bp, which had a 99.68% homology to the E. miyagawai accessed in GenBank. The morphological findings were in agreement with molecular identification. Conclusion E. miyagawai infection is common in domestic ducks in Wuhu area, and the mitochondrial Cox1 gene is a feasible marker of intra- and inter-species molecular identification of Echinostoma.
