Molecular identification of Tricula spp. and the parasitized trematode cercariae in schistosomiasis-endemic areas of Yunnan Province
10.16250/j.32.1374.2019187
- VernacularTitle:云南省血吸虫病流行区一种拟钉螺及其寄生吸虫尾蚴的分子鉴定
- Author:
Chun-Hong DU
1
;
Shan LÜ
2
,
3
;
Yun ZHANG
1
;
Shi-Zhu LI
2
;
Meng-Tao XIONG
1
;
Zhi-Hai HE
1
;
Zhi-Hua LI
4
;
Ming-Shou WU
1
;
Jia-Yu SUN
1
;
Yin-Ben REN
4
;
Chun-Qiong CHEN
1
;
Qiong GU
4
;
Yun-Song WANG
1
;
Yi DONG
1
Author Information
1. Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China
2. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, China
3. Co-first author
4. Xiangyun Station of Schistosomiasis Control, Yunnan Province, China
- Publication Type:Journal Article
- Keywords:
Tricula spp.;
Oncomelania snail;
Trematode;
Cercaria;
16S rRNA gene;
COI gene;
28S rDNA gene;
ND1 gene;
Schistosomiasis;
Yunnan Province
- From:
Chinese Journal of Schistosomiasis Control
2020;32(2):159-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize a species of the genus Tricula and parasitized trematodes in schistosomiasis-endemic areas of Yunnan Province using a molecular analysis, so as to understand their taxonomic positions. Methods Tricula spp. and Oncomelania snails were collected from Xiangyun County, Yunnan Province, and cercaria parasitizing snails were observed using crushing followed by microscopy. Cercaria parasitizing Tricula snails at various morphologies were sampled using a shedding method. Genomic DNA was extracted from snail soft tissues and cercariae, and the 16S rRNA, COI, 28S rDNA genes in snails and the ND1 and 28S rDNA genes in cercariae were amplified using a PCR assay and sequenced. The species of Tricula snails and their parasitized trematodes was characterized using sequence alignment and phylogenetic analysis. Results Among 382 Tricula snails detected, there were three types of trematode cercariae found, including the non-forked (20.94%, 80/382), double-forked (3.40%, 13/382) and swallow shapes (7.07%, 27/382). Sequence and phylogenetic analyses showed that the 16S rRNA, COI and 28S rDNA gene sequences of this species of Tricula had high homology to those in Delavaya dianchiensis, and were clustered in a branch. Sequencing analysis of the ND1 and 28S rDNA genes revealed that the non-forked cercariae belonged to the family Pleu- rogenidae, the swallow-shaped cercariae belonged to the family Opecoelidae, and the double-forked cercariae belonged to another species of the genus Schistosoma that was different from S. sinensium and S. ovuncatum. Conclusion The species and taxonomy of Triculla spp. and their parasitized trematodes are preliminarily determined in schistosomiasis-endemic areas of Yunnan Province; however, further studies are required to investigate the more definite taxonomy and pathogenicity.
