Correlation Study of Color Difference Values and Active Constituent Contents in Crude and Processed Zingiber officinale
- VernacularTitle:干姜及其炮制品色差值与活性成分含量的相关性研究
- Author:
Huajian LIN
1
;
Zihao ZHANG
1
;
Jiang MENG
1
;
Shumei WANG
1
Author Information
1. School of Traditional Chinese Medicine，Guangdong Pharmaceutical University/Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica，State Administration of Traditional Chinese Medicine/Engineering Technology Research Center for Chinese Materia Medica Quality of Guangd ong Universities，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Crude Zingiber officinale;
Processed product;
HPLC;
Chromatric aberration meter;
Active constituent;
Content
- From:
China Pharmacy
2020;31(10):1197-1202
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To evaluate the correlation between color difference values and active constituent contents of crude and processed Zingiber officinale . METHODS ：HPLC method was adopted to determint the content of 6 active constituents. The color difference values of crude and processed Z. officinale [lightness（L＊），red-green axis component （a＊），yellow-blue axis component（b＊）] were determined by chromatic aberration meter . SPSS 24.0 software was adopted for the correlation analysis between color difference values and active constituent contents. RESULTS ：The linear range of zingiberone ，6-gingerol， 8-gingerol， 6-shogaol， diacetoxy-6-gingerol and 10-gingerol were 2.65-105.90， 10.15-406.00， 4.87-194.80， 5.28-211.20， 6.14-245.70，7.02-280.80 μg/mL（r＞0.999）. The limits of quantification were 7.46，13.68，14.37，16.62，17.03，17.99 ng，and the limits of detection were 2.24，4.11，4.31，4.99，5.11，5.40 ng，respectively. RSDs of precision ，stability，and repeatability tests were all lower than 3％. The average recovery rates were 101.34％，102.14％，101.22％；103.12％，103.74％，103.54％；103.06％，properties critical for cell migration and invasion. induced EMT through downregulation of NF-κB-Snail sig- naling in breast cancer cells enchymal transition and activation of TLR 4/JNK signal - 98.55％，99.43％；99.36％，103.51％，101.21％；100.85％，99.42％，99.60％；100.39％，97.69％，103.84％（RSD were all lower than 3％，n＝3），respectively. The contents of them were 0-0.66，0.06-7.57，0.03-1.45，0.29-3.47，0.15-2.85，0.04-2.83 mg/g， respectively. L＊ and b＊ values were negative correlated with the processing degree of Z. officinale significantly（P＜0.01），a＊ showed a significantly positive correlation with the processing degree （P＜0.05）. L＊and b＊ values showed a significantly negative correlation with the content of zingiberone before and after processing ，but positively correlated with the other five components （P＜0.01）. a＊ showed a significantly positive correlation with the content of zingiberone ，but had no correlation with other five components（P＞0.05）. The crude and processed Z. officinale were positive correlated with the content of zingiberone ，negatively correlated with other five components （P＜0.01）. CONCLUSIONS ：There is a certain correlation between the color difference values of crude and processed Z. officinale and the contents of their active constituents. With the deepening of the processing ，a＊ values is increased ，L＊ values and b＊ values is decreased ；the content of zingiberone increases ，the contents of 6-gingerol， 8-gingerol，6-shogaol，diacetoxy-6-gingerol，10-gingerol reduce.
