Influence of concentrations of CaCl2 on hemolytic activity of candida strains
10.12016/j.issn.2096-1456.2017.08.004
- Author:
WAN Lei
1
;
LU Haibin
1
;
LUO Gang
2
Author Information
1. Department of Periodontology and Implantology Stomatological Hospital Southern Medical University
2. Key Laboratory of Oral Medicine Guangzhou Institute of Oral Disease Stomatology Hospital of Guangzhou Medicial University
- Publication Type:Journal Article
- Keywords:
Candida species;
Virulence factors;
Hemolytic activity;
Electrolytes;
CaCl2
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(8):494-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of different concentrations of CaCl2 on the hemolytic activity of candida strains.
Methods :A total of 25 candida strains were selected, including 21 candida strains recovered from clinical specimens, and 4 reference strains of C. albicans (ATCC 90028) (American Type Culture Collection, Manassas, VA, USA), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), and C. tropicalis (ATCC 13803) respectively. CaCl2 was added at concentrations of 0%, 0.5%, 1% and 2.5 g % (wt/vol). Plates were cultured at 37 ℃ in a 5% CO2 atmosphere for 24-72 h, and the hemolytic index (Hi) was analyzed.
Results :comparsions of the hemolytic indices among the groups treated with 0.5% CaCl2 (Hi = 2.247 ± 0.079), 1% CaCl2 (Hi = 2.013 ± 0.092), 2.5% CaCl2 (Hi = 2.150 ± 0.066) and the control (Hi = 2.749 ± 0.103) reached statistical significance (P < 0.001).
Conclusion:CaCl2 may produce a decrease activity in the hemolysis of candida species.
- Full text:氯化钙浓度对念珠菌溶血活性的影响.pdf
