Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology
- VernacularTitle:Box-Behnken设计-响应面法优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺
- Author:
Zhoufeng HUANG
1
,
2
;
Xiaoxi HU
1
;
Jianyou HUANG
1
;
Xiao TAN
1
;
Guoshou LU
1
;
Xinqiao LIANG
3
Author Information
1. Guangxi Zhuang Autonomous Region Institute of Traditional Medical and Pharmaceutical Sciences，Nanning 530022，China
2. Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards，Nanning 530022，China
3. College of Pharmacy，Guangxi University o f TCM，Nanning 530022，China
- Publication Type:Journal Article
- Keywords:
Desmodium caudatum;
Citrusinol;
Aqueous two-phase extraction;
Ultrasonic extraction;
Box-Behnken design
- From:
China Pharmacy
2020;31(9):1085-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To optimize ultrasonic-assisted ethanol-（NH4）2SO4 aqueous two-phase extraction technology of citru- sinol from Desmodium caudatum . METHODS ：Using the content of citrusinol as indexes ，with ethanol volume fraction ， solid-liquid ratio ，（NH4）2SO4 addition amount ，ultrasonic time and ultrasonic temperature as factors ，based on the single factor tests，Box-Behnken design-response surface methodology was used to optimize the extraction technology of citrusinol. RESULTS ： The optimized extraction technology of citrusinol included that ethanol volume fraction was 95.35％，the solid-liquid ratio was 1∶50.35 （g/mL），（NH4）2SO4 addition amount was 4.49 g，ultrasonic time was 48.7 min，ultrasonic temperature was 57.6 ℃. In 3 times of validation tests ，the extraction rates of citrusinol were 0.637 8，0.638 4，0.625 4 mg/g，respectively，which was close to predicted value（0.630 5 mg/g）. CONCLUSIONS ：The optimized ultrasonic-assisted ethanol- （NH4）2SO4 aqueous two-phase extraction technology is stable and feasible ，and can be used for the extraction of citrusinol from D. caudatum .
