Simultaneous Determination of 7 Effective Components in Yao Medicine Yueli Yaomi Spray by QAMS
- VernacularTitle:一测多评法同时测定瑶药月里瑶秘喷雾剂中7种有效成分的含量
- Author:
Jingzhi ZHANG
1
;
Danqing ZHAO
2
;
Zhengrong WANG
1
,
2
;
Jinyan JIA
3
;
Lin YANG
3
;
Shengsheng ZHANG
4
;
Xinlei LIU
1
;
Qing WU
4
;
Wei PAN
1
Author Information
1. School of Clinical Laboratory Science，Guizhou Medical University，Guiyang 550004，China
2. Guizhou Provincial Prenatal Diagnosis Center，the Affiliated Hospital of Guizhou Medical University，Guiyang 550004，China
3. Guizhou Hongyu Pharmaceutical Co.，Ltd.，Guiyang 550001，China
4. Guizhou Key Laboratory of Mountainous Environment Information System and Environment Protection/Analysis and Measurement Center，Guizhou Normal University，Guiyang 550001，China
- Publication Type:Journal Article
- Keywords:
QAMS;
Yao medicine;
Yueli yaomi spray;
GC;
Quality control
- From:
China Pharmacy
2020;31(9):1044-1049
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish QAMS method for simultaneou s determination of 7 effective components in Yao medicine Yueli yaomi spray ，such as α-cyperone，α-pinene，β-pinene，limonene，β-elemene，caryophyllene oxide and ligustilide ， so as to provide method reference for the quality control of the preparation. METHODS ：GC method was adopted. The determination was performed on DB- 1701P capillary column ，using nitrogen as carrier gas. The temperature of the hydrogen flame ion detector was 240 ℃. The temperature was programmed ，the inlet temperature was 240 ℃，the injection volume was 1 μL and the split ratio was 20 ∶ 1. Using limonene as internal reference ，the relative correction factors of other 6 components were calculated，the contents of them were calculated with relative correction factors ，and then compared with the results of internal standard method （using naphthalene as internal standard ）. RESULTS ：The mass concentration linear range of α-cyperone， α-pinene，β-pinene，limonene，β-elemene，caryophyllene oxide and ligustilide were 0.008 9-1.110 0，0.028 3-3.540 0，0.020 5- 2.560 0，0.023 0-2.880 0，0.016 3-2.035 0，0.013 1-1.640 0，0.008 3-1.040 0 mg/mL（all r＞0.999 0）；the limits of quantification were 0.005 6，0.013 1，0.011 4，0.018 6，0.010 8，0.008 9，0.004 5 mg/mL；the detection limits were 0.001 9，0.004 1，0.003 7， 0.006 2，0.003 5，0.002 9，0.001 5 mg/mL；RSDs for precision ，stability（24 h），and repeatability tests were all less than 2％ （n＝5 or n＝6）； the average recoveries were 98.48％ ， 014） 101.37％，97.96％，99.80％，102.79％，97.77％，102.14％， and RSDs were all lower than 2％（n＝9），respectively. The average relative correction factors of α-cyperone，α-pinene， β-pinene，β-elemene，caryophyllene oxide and ligustilide were 1.045 8，0.621 0，0.488 5，0.382 9，0.708 9，0.956 9 respectively，and the RSDs were all lower than 2％（n＝6）. There wa s no statistical significance in contents of 7 components between QAMS method and internal standard method （P＞0.05）. CONCLUSIONS ：The established QAMS method is simple ， accurate，stable and reproducible ，and can be used for simultaneous determination for 7 components in Yueli yaomi spray.
