Research on the fingerprint and three active components assay in Shenshuaining granules by HPLC
10.12206/j.issn.1006-0111.201911005
- VernacularTitle:肾衰宁颗粒指纹图谱研究及3种有效成分含量测定
- Author:
Xinhua SONG
1
;
Xujiao CHEN
2
;
Fengyi DENG
1
;
Shouhong GAO
3
;
Hui PENG
4
Author Information
1. College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China.
2. Department of Nephrology, School of Medicine, 2nd Affiliated Hospital, Zhejiang University, Hangzhou 310002, China.
3. Department of Pharmacy, Changzheng Hospital Affiliated to Naval Medical University, Shanghai 200003, China.
4. People’s Hospital of Yichun City, Yichun 336000, China.
- Keywords:
Shenshuaining granules;
HPLC;
fingerprinting;
content determination
- From:
Journal of Pharmaceutical Practice
2020;38(3):259-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprint spectrum and assay three active components (hesperidin, salvianolic acid B and chrysophanol) in Shenshuaining granule by HPLC method. Methods The chromatographic separation was achieved on SunFireTM C18 column with acetonitrile-0.1% formic acid aqueous solution as mobile phase. Gradient elution program was applied with flow rate of 1.0 ml/min, detection wavelength at 254 nm and the column temperature at 25 ℃. The fingerprint spectrum was established and three active components in Shenshuaining granule were assayed. Results There were 22 common peaks on the fingerprints after analyzing chromatograms from 10 batches of Shenshuaining granules. Good fingerprint similarities (≥0.9) between different batches and the control chromatogram were found. This method has great repeatability, stability and precision, which meets all the assay requirements. Conclusion A simple and reliable HPLC method was developed, which is suitable for the fingerprint establishment of Shenshuaining granules. It provides a method for the quality control of Shenshuaining granules.
