Rapid identification of chemical constituents in serum and plasma of Fuzheng Huayu capsules by UHPLC-TOF/MS
10.12206/j.issn.1006-0111.201906027
- VernacularTitle:基于UHPLC-Q-TOF/MS技术对扶正化瘀胶囊入血成分的快速鉴别
- Author:
Xinrui XING
1
,
2
;
Qi CAO
3
;
Si CHEN
4
;
Zhenyu ZHU
3
Author Information
1. Department of Pharmacy, Naval Medical University, Shanghai 200433, China
2. Linyi Inspection and Testing Center, Linyi 276037, China.
3. Department of Pharmacy, Naval Medical University, Shanghai 200433, China.
4. No. 210 Hospital of Chinese People's Liberation Army, Dalian 116000, China.
- Keywords:
Fuzheng Huayu capsule;
identification;
chemical composition in plasma and serum
- From:
Journal of Pharmaceutical Practice
2020;38(3):250-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the blood components of Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Methods ACQUITY UPLCHSS T3 (2.1 mm × 100 mm, 1.8 μm) was used to chromatographic separation; mobile phase was 0.1% formic acid aqueous solution (A) −0.1% formic acid acetonitrile solution (B). The gradient elution conditions included: 0−3 min, 2% B; 3−18 min, 2%−50% B; 18−22 min, 50%−95% B; 22−25 min, 95% B. The equilibration time was 10 min, the flow rate was 0.40 ml/min, and the analysis time was 25 min. The mass spectrometry was characterized by electrospray ionization by a positive-negative ion mode scan with a range of 100-1 100 m/z. Results 49 components were identified in the serum samples at one time, of which 4 were positive and negative ion modes. Conclusion The blood components of Fuzheng Huayu capsule were clarified by this method, which enriched the scientific connotation of Fuzheng Huayu capsule, and laid the foundation for the in-depth study of the compound.
