Improvement of impaired endothelial progenitor cell functions by in vitro drug interference in DOCA-salt hypertensive mice
10.12206/j.issn.1006-0111.201912074
- VernacularTitle:药物体外干预改善盐敏感性高血压小鼠内皮祖细胞功能研究
- Author:
Liuwenxin SHANG
1
;
Xin SUN
2
;
Cheng PENG
2
;
Hehui XIE
2
;
Fengyuan CHEN
2
;
Chuan ZHANG
1
Author Information
1. Department of Authentication of Chinese Medicines, Naval Medical University, Shanghai 200433, China.
2. School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
- Keywords:
endothelial progenitor cells;
hypertension;
tetrahydrobiopterin;
superoxide dismutase;
N(G)-nitro-L-arginine
- From:
Journal of Pharmaceutical Practice
2020;38(3):221-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tetrahydrobiopterin (BH4), superoxide dismutase-polyethylene glycol (PEG-SOD) and N(G)-nitro-L-arginine (L-NNA) on impaired endothelial progenitor cell (EPC) functions in DOCA-salt hypertensive mice. Methods DOCA-salt hypertension was created and systolic blood pressure was measured by tail-cuff methods. EPC was isolated from bone marrow of mice and characterized by flow cytometry and fluorescence microscopy. EPC of DOCA-salt mice was incubated with BH4, PEG-SOD, and L-NNA for 24 h, then in vitro EPC function assays were performed. Results Compared with control group, systolic blood pressure was significantly increased in DOCA-salt mice. Both EPC adhesion and angiogenesis functions were impaired in DOCA-salt mice compared to control animals, which were reversed by incubation with BH4, PEG-SOD and L-NNA. Conclusion BH4, PEG-SOD and L-NNA rescued the impairments from EPC functions in DOCA-salt hypertensive mice.
