Anti-inflammatory study on 9,9'-O-di-(E）-feruloyl-meso-5,5'-dimethoxysecoisolariciresinol (LCA), an active ingredient in Litsea cubeba (Lour.) Pers
10.12206/j.issn.1006-0111.201912159
- VernacularTitle:豆豉姜活性成分9,9’-O-二-(E)-阿魏酰基-内消旋-5,5’-二甲氧基开环异落叶松树脂酚（LCA）抗炎作用研究
- Author:
Luyao YU
1
;
Dan JIA
2
;
Hui SHEN
2
;
Xiaolei SUN
2
;
Luping QIN
2
,
3
;
Ting HAN
2
Author Information
1. School of Life Science, Shanghai Normal University, Shanghai 200234, China.
2. Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
3. School of Life Science, Shanghai Normal University, Shanghai 200234, China
- Keywords:
LCA;
anti-inflammatory;
ear swelling;
granuloma of cotton ball;
RAW264.7 cell
- From:
Journal of Pharmaceutical Practice
2020;38(3):216-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-inflammatory effects of LCA, which is the active component from Litsea cubeba (Lour.) Pers. Methods Pharmacodynamic evaluations of ear swelling and cotton ball granuloma models were used in animal experiments. In vitro experiment, lipopolysaccharide (LPS) stimulated monocyte macrophage RAW264.7 cells were used to evaluate the anti-inflammatory activity of LCA by detecting the secretions of nitric oxide (NO), tumor necrosis factor-α (TNF-α), the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. Results In ear swelling experiment, the extent of ear swelling was significantly lower in the LCA treated group than the model group. The inhibition rate was greater in the high LCA concentration group than the positive drug group. In addition, LCA reduced the weight of cotton ball granuloma markedly. At the cell level, LCA significantly inhibited the secretions of NO, TNF-α and reduced the expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. Conclusion LCA has significant anti-inflammatory effects in vitro and in vivo. The further studies are warranted for the development of anti-inflammatory drugs.
