Expression of lncRNA LINC01001 in breast cancer and its effect on proliferation of MCF-7 cells
10.3872/j.issn.1007-385x.2018.02.009
- VernacularTitle:长链非编码RNALINC01001在乳腺癌中的表达及其对MCF-7细胞增殖 的影响
- Author:
MEI Hong
1
,
2
;
LI Changen
1
,
2
;
YANG Liang
1
,
2
;
GAO Yingfei
1
,
2
Author Information
1. DepartmentofThyroidand Breast Surgery,Affiliated People&rsquo
2. s Hospital of Hubei University of Medicine, Shiyan 442000, Hubei, China
- Publication Type:Journal Article
- Keywords:
breast cancer;
long non-coding RNA(lncRNA);
microRNA(miRNA);
CDKN1A;
proliferation
- From:
Chinese Journal of Cancer Biotherapy
2018;25(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the expression of long non-coding RNA LINC01001 in breast cancer tissues and its effect on the proliferation of breast cancer MCF-7 cells. Methods: The expression levels of lncRNA LINC01001 were analyzed in 12 cases of cancer and para-cancer tissues from breast cancer patients, who underwent surgical resection in Affiliated People’s Hospital of Hubei University of Medicine from March 2016 to June 2017. The plasmid over-expressing LINC01001 was transfected into human breast cancer MCF-7 cells. The cell cycle distribution and proliferation ability of MCF-7 cells were detected by flow cytometry and MTT assay, respectively. The mRNA expressions of miR-485-5p and CDKN1A mRNA were detected by qRT-PCR, and the protein expressions of CDKN1A, CDK4, CDK6 and Cyclin D1 were detected by Western blotting. Results: The expression level of lncRNA LINC01001 in breast cancer tissues was lower than that in para-cancer tissues (P<0.01). LINC01001 recombinant plasmid transfection significantly inhibited cell cycle progression (P<0.05) and cell proliferation (P<0.05) of MCF-7 cells. qRT-PCR showed that the expression level of miR-485-5p was decreased (P<0.01) and the expression level of CDKN1A mRNA was increased (P<0.01) after over-expressing LINC01001. Western blot results confirmed that over-expression of LINC01001 could promote the expression of CDKN1A protein, but decrease the expressions of CDK4, CDK6 and Cyclin D1 proteins. Conclusion: The expression of LINC01001 in breast cancer tissues was decreased. LINC01001 may down-regulate the expression of miR-485-5p to up-regulate the expression of CDKN1A, and further to inhibit the proliferation of breast cancer MCF-7 cells, providing experimental basis for the clinical application of lncRNA.
- Full text:20180209.pdf
