Expression of plasma-cytoma variant translocation gene 1 in ovarian cancer and its malignant biologic behavior
10.3872/j.issn.1007-385x.2018.02.008
- VernacularTitle:浆细胞瘤多样异位基因1在卵巢癌组织中的表达及其对SKOV3细胞恶 性生物学行为的影响
- Author:
LI Xiaozhen
1
;
REN Chenchen
1
;
LIU Ling
1
;
LIU Jiaxi
1
Author Information
1. Department of Gynecology, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China
- Publication Type:Clinical Trial
- Keywords:
ovarian cancer;
plasma-cytoma variant translocation gene 1(PVT1);
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(2):153-157
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effects of plasma-cytoma variant translocation gene 1 (PVT1) on proliferation, migration and invasion of ovarian cancer SKOV3 cells. Methods: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression of PVT1 in 32 pairs of ovarian cancer tissues and corresponding para-carcinoma tissues. The effects of PVT1 on proliferation, migration and invasion of ovarian cancer cells were studied by CCK-8, scratch wound healing assay and Transwell assay. Results: The expression level of PVT1 in SKOV3 cells and ovarian cancer tissues was significantly increased (all P<0.01). The expression level of PVT1 was correlated with histological grade, FIGO stage and lymph node metastasis in patients with ovarian cancer (P<0.05 or P< 0.01).After transfection with PVT1 siRNAfor 36, 48 h, expression of PVT1 was significantly decreased in SKOV3 cells; and the inhibition of PVT1 expression could decrease the proliferation ability and inhibit the migration and invasion of SKOV3 cells (P<0.05 or 0.01). Conclusion: LncRNA PVT1 was highly expressed in ovarian cancer. Down-regulation of PVT1 could inhibit the proliferation, migration and invasion of ovarian cancer SKOV3 cells.
- Full text:20180208.pdf
