Characteristics and clinical significance of osteogenic differentiation of mesenchymal stem cells derived from bone marrow in patients with myelodysplastic syndromes
10.3872/j.issn.1007-385x.2018.02.007
- VernacularTitle:骨髓增生异常综合征患者骨髓来源间充质干细胞的成骨分化特点及其 临床意义
- Author:
PANG Yanbin
1
;
REN Jie
2
;
FAN Lixia
1
;
WANG Jing
1
;
ZHAO Songying
1
;
XUE Hua
1
;
HUA Luoming
1
;
DU Xin
3
Author Information
1. 1. Department of Hematology,Afilliatied Hospital of Hebei Universtity，Baoding 071000, Hebei, China
2. 2. Department of Internal Medicine, Yixian County Hospital of Hebei, Yixian 074200, Hebei, China
3. 3. Department of Hematology, Guangdong General Hospital / Guangdong Academy of Medical Sciences, the First Clinical Hospital of Medicine School of South China University, Guangzhou 510080, Guangdong, China
- Publication Type:Clinical Trial
- Keywords:
myelodysplastic syndromes(MDS);
mesenchymal stem cell(MSC);
osteogenic differentiation
- From:
Chinese Journal of Cancer Biotherapy
2018;25(2):148-152
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the osteogenic differentiation characteristics of mesenchymal stem cell (MSC) derived from bone marrow in patients with myelodysplastic syndromes (MDS) and its clinical significance. Methods: Bone marrow samples from 30 cases of newly diagnosed untreated MDS patient atAffiliated Hospital of Heibei University were collected for this study. MSCs from MDS patients and normal subjects were isolated and cultured, and morphological characteristics of MSCs were observed in vitro; under proper conditions, MSCs were induced to differentiate into osteoblasts and adipocytes; The formation of calcium nodules at 14th day after osteogenic differentiation was observed by alizarin red staining; mRNA expressions of osteogenic differentiation transcription factors Ostefix and RUNX2 in undifferentiated MSCs, as well as the mRNAexpression of Jagged-1, which involved in the transformation from hematopoietic cells into leukemic cells, were detected by quantitative PCR. Results: The MSCs derived from patients with MDS were characterized with increased cell volume and decreased differentiation potential. Compared with the control group, the expression levels of osteogenic differentiation transcription factors Osterix and RUNX2 were significantly decreased (P < 0.05). Alizarin red staining showed that the content of calcium nodules in MDS group was significantly less than that in the normal control group, while the expression level of Jagged-1 was significantly higher (P < 0.05). Conclusion: MSCs derived from bone marrow of MDS patients showed significant increased cell volume, decreased differentiation potential and elevated Jagged-1 expression; all of these might play important roles in the .hematopoietic failure and progression to acute myeloid leukemia in MDS patients.
- Full text:20180207.pdf
