IL-18 over-expression inhibits proliferation of human colorectal cancer HCT-116 cells
10.3872/j.issn.1007-385x.2018.02.006
- VernacularTitle:过表达I L - 1 8 抑制人结直肠癌细胞HCT-116的增殖
- Author:
CHEN Yanan
1
;
YANG Ru
1
;
XU Zhishan
1
;
WU Minna
2
;
LU Ping
3
;
ZHONG Genshen
1
Author Information
1. 1a. Research Centre of Life Science and Technology
2. 2. Department of Microbiology, College of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China
3. 1b. Department Oncology，the FirstAffiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China
- Publication Type:Journal Article
- Keywords:
colorectal cancer（CRC）;
interlukin-18（IL-18）;
proliferation;
transplanted tumor
- From:
Chinese Journal of Cancer Biotherapy
2018;25(2):142-147
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effects of interleukin-18 over-expression on the in vitro and in vivo proliferation of human colorectal cancer (CRC) HCT-116 cells. Methods: A recombinant lentivirus vector containing human IL-18 gene fragment was constructed. Then theCRC HCT-116 cell line stably expressing human IL-18 (HCT-116/IL-18) was obtained by recombinant lentivirus transfection. In vitro proliferation of HCT-116/IL-18 cells and wild-type HCT-116 cells was determined by CCK-8 method. The expressions of IL-18, Cyclin D1, proliferating cell nuclear antigen (PCNA) and DNA damage repair enzyme (PARP) were detected by Western blotting. HCT-116 and HCT-116/IL-18 cells were inoculated into left and right axillas of Balb/c nude mice, respectively. Then the tumorigenicity and the growth of transplanted tumor were observed. The expressions of IL-18 and PCNAin xenograft tissues were detected by immunohistochemistry analysis. Results: IL-18 gene over-expression in HCT-116 cells could delay the proliferation of HCT-116 cells (P<0.05 or P<0.01). PARP expression was increased significantly and PCNA, Cyclin D1 expression were decreased in HCT-116/ IL-18 cells as compared to that of HCT-116 cells (P<0.01).The tumorigenicity of HCT-116/IL-18 cell was significantly decreased in nude mice with a tumor-formation rate of 43%; Compared with control group, HCT-116/IL-18cell line had a longer tumorigensis time, slower growth and smaller tumor volume; moreover, PCNAprotein expression was down-regulated in HCT-116/IL-18 xenograft tissuesas shown by immunohistochemistry analysis (P<0.01). Conclusion: IL-18 over-expression inhibited the growth and proliferation of HCT-116 cells both in vitro and in vivo, and the mechanism might berelated with IL-18 regulating cell cycle and promoting DNA damage.
- Full text:20180206.pdf
