Effects of IL-7 and IL-21 modified NK-92MI cells on themselves and T cells from normal human peripheral blood
10.3872/j.issn.1007-385x.2018.03.012
- VernacularTitle:IL-7和IL-21修饰的NK-92MI细胞对其自身及正常人外周血T细胞的 影响
- Author:
ZHANG Ping
1
;
LI Yafen
2
;
AN Gangli
1
;
YANG Lin
3
Author Information
1. 1.The Cyrus Tang Hematology Center, Medical department, Soochow University, Suzhou 215123, Jiangsu,China
2. 2. Persongen Bio Therapeutics (Suzhou) Co., Ltd., Suzhou 215123, Jiangsu, China
3. 1.The Cyrus Tang Hematology Center, Medical department, Soochow University, Suzhou 215123, Jiangsu,China；2. Persongen Bio Therapeutics (Suzhou) Co., Ltd., Suzhou 215123, Jiangsu, China
- Publication Type:Clinical Trial
- Keywords:
NK-92MI cell；NK-92MI/IL-21 cell；NK-92MI/IL-7&21cell；PBMC；cytotoxicity
- From:
Chinese Journal of Cancer Biotherapy
2018;25(3):281-287
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective：To investigate whether the proliferation and cytotoxicity of NK-92MI cells can be improved by IL-7 and IL-21 genes modification, and determine the effects of this genetically modified NK-92MI cells on T cells from normal human peripheral blood. Methods：IL-7 and IL-21 gene fragments were constructed into electroporation vector by genetic engineering method, and NK92MI/IL-21 and NK-92MI/IL-7&21 cells were constructed by electroporation transfection. The in vitro proliferation and cytotoxicity of NK-92MI, NK-92MI/IL-21 and NK-92MI/IL-7&21 cells were measured by cell count and flow cytometry assays. Then, normal human PBMCs were co-cultured with NK-92MI, NK-92MI/IL-21 and NK-92MI/IL-7&21 cells in vitro respectively, and the phenotype change of T cells was measured by flow cytometry. In addition, the cytotoxicity between the activated T cells and three NK-92MI cell lines (NK-92MI, NK-92MI/IL-21 and NK-92MI/IL-7&21 cells) as well as the cytotoxicity of the three NK-92MI cells on tumor cells after co-incubation with activated T cells were detected. Results: NK-92MI/IL-21 cell line (highly expressing IL-21) and NK-92MI/IL-7&21 cell line (highly expressing both IL-7 and IL-21) were successfully constructed. The toxicity of NK-92MI, NK-92MI/IL-21 and NK92MI/IL-7&21 cells on Jurkat and K562 cells showed no difference, while the proliferation of NK-92MI/IL-21 and NK-92MI/IL-7&21 cells was increased compared with NK-92MI cells. Furthermore, NK-92MI/IL-21 and NK-92MI/IL-7&21 cells promoted the activation of T cells to a certain degree, and the activated T cells showed merely no cytotoxicity on NK-92MI, NK-92MI/IL-21 and NK-92MI/IL7&21 cells; Meanwhile, the activated T cells did not affect the cytotoxicity of the three NK cells (NK-92MI, NK-92MI/IL-21, and NK92MI/IL-7&21 cells) on K562 cells under their co-existence. Conclusion: The in vitro proliferation of NK-92MI/IL-21 and NK-92MI/ IL-7&21 cells were enhanced after gene modification, which could also stimulate and promote the activation of T cells from peripheral blood. The cytotoxicity assay showed that the activated T cells had no cytotoxicity on NK-92MI, NK-92MI/IL-21, and NK-92MI/IL-7& 21 cells. Meanwhile, the presence of the activated T cells did not affect the cytotoxicity of NK-92MI cells.
- Full text:20180312.pdf
