Effect of Tim-4 on invasion and migration of SiHa cervical cancer cell and its underlying mechanism
10.3872/j.issn.1007-385x.2018.03.010
- VernacularTitle:Tim-4促进子宫颈癌细胞SiHa侵袭与迁移及其可能机制
- Author:
JIANG Jie
1
;
YANG Mingghao
1
;
JIANG Zhaoling
1
;
WANG Guoyan
1
;
XIU Yan
1
;
ZHAO Peiqing
1
Author Information
1. (Department of Clinical Laboratory, Yantai Affiliated Hospital of Binzhou Medical University,Yantai 246199, Shandong, China
- Publication Type:Journal Article
- Keywords:
Tim-4;
cervical cancer;
EMT;
invasion;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(3):270-274
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of Tim-4 on invasion and migration of cervical cancer cells and its underlying mechanisms. Methods：The expression levels of Tim-4 in cervical cancer cell lines Siha, Hela and cervical epithelial immortalized cell line H8 were detected by Real-time PCR. The Tim-4 lentiviral vector was transfected into Siha cell line. The over-expression of Tim-4 in Siha cell line was detected by fluorescence microscopy. The effects of Tim-4 on the invasion and migration of cervical cancer cell line were detected by Transwell and scratches methods. The changes of MMP2, MMP9, E-cadherin and N-cadherin in Siha cells were detected by Western blotting. Results：The expression of Tim-4 was higher in Siha and Hela cell lines compared to that in the H8 cell line. The Siha cell line burdening Tim-4 lentiviral vector was successfully constructed. Over-expression of Tim-4 significantly inhibited the migration and invasion of cervical cancer cell line, and affected the expression of MMP2, MMP9, N-cadherin and E-cadherin. Conclusion：Over-expression of Tim-4 promotes the invasion and migration by regulating the EMT transformation in cervical cell carcinoma.
- Full text:20180310.pdf
