The effect of FBXW7 gene point mutation to colorectal cancer HCT-116 cells’proliferation, migration, invasion, and its apoptosis resistance
10.3872/j.issn.1007-385x.2018.03.009
- VernacularTitle:FBXW7基因点突变对结直肠癌HCT-116细胞的增殖、迁移、侵袭和抗 凋亡能力的影响
- Author:
ZHANG Baichuan
1
;
CHENG Yong
1
;
WANG Xiangfeng
2
;
TANG Kang
1
;
WANG Wuyi
1
Author Information
1. 1.Department of Gastrointestinal Surgery，the 1st Hospital Affiliated to Chongqing Medical University，Chongqing 400016，China
2. 2. Department of General Surgery,Daping Hospital and the Research Institute of Surgery, Third Military Medical University, Chongqing 400016，China
- Publication Type:Journal Article
- Keywords:
FBXW7 gene;
gene mutation;
colorectal cancer;
HCT-116 cell;
ubiquitin-proteasome system
- From:
Chinese Journal of Cancer Biotherapy
2018;25(3):263-269
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the impact of FBXW7 gene mutation on cell proliferation, apoptosis, migration, and invasion processes of colorectal cancer HCT-116 cell line. Methods: Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line; the FBXW7 protein expression level in HCT-116 strains after transfection was detected by Western blotting. Subsequently, cell proliferation capacity was tested by CCK-8 assay; tumor cell colony formation ability was tested by HTCA; cell apoptosis function was tested by FCM; cell migration and invasion were tested by scratch assay and Transwell assay, respectively. Results: Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group (strains transfected with mutant-type HBXW7), negative-control (strains transfected with empty plasmids), and blank-control (strains untransfected) (all P<0.05). Compared with the other groups, strains transfected with wildtype HBXW7 exhibited significantly reduced proliferation, colony formation, migration and invasion ability (all P<0.05), but obviously increased apoptosis rate (P<0.05). Conclusion： ：FBXW7 gene mutation can down-regulate its protein expression, and further promote the proliferation, migration and invasion as well as inhibit the apoptosis of HCT-116 cells.
- Full text:20180309.pdf
