Effect of ursolic acid combined with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells
10.3872/j.issn.1007-385x.2018.03.007
- VernacularTitle:乌苏酸联合吉西他滨对胰腺癌PANC-1细胞增殖和凋亡的影响
- Author:
JIANG Sen
1
;
ZHENG Xi
1
,
2
;
HE Yan
3
;
TINA Liao
4
;
ZHANG Kun
5
Author Information
1. 1. Laboratory of Natural Medicinal Chemistry and Green Chemistry, Guangdong University of Technology, Guangzhou 510006, Guangdong, China
2. 2. Rutgers Cancer Institute of New Jersey, the State University of New Jersey, Piscataway 08854，N J，U SA
3. (1. Laboratory of Natural Medicinal Chemistry and Green Chemistry, Guangdong University of Technology, Guangzhou 510006, Guangdong, China
4. 2. Rutgers Cancer Institute of New Jersey, the State University of New Jersey, Piscataway 08854，N J，U SA
5. 1. Laboratory of Natural Medicinal Chemistry and Green Chemistry, Guangdong University of Technology, Guangzhou 510006, Guangdong, China；3 Wuyi University, Jiangmen 529020, Guangdong, China
- Publication Type:Journal Article
- Keywords:
pancreatic cancer;
ursolic acid;
gemcitabine;
proliferation;
migration;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(3):252-257
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To study the effects of ursolic acid cooperated with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells. Methods: Human pancreatic cancer cell line PANC-1 was cultured in vitro with ursolic acid and gemcitabine respectively; and MTT assay was used to determine the IC50 of ursolic acid and gemcitabine, thus obtaining the best drug concentration. Ursolic acid (2 µmol/L) and gemcitabine (0.2 µmol/L) alone or in combination was used to treat PANC-1 cells; trypan blue assay was used to test cell viability, and PI staining was used to examine the cell apoptosis; wound healing was used to detect the proliferation and migration of PANC-1 cells. The protein expressions of P-JNK, Bcl-2, IL-6, P-Stat 3, NF-κB and Cox-2 in cells of each treatment group were detected using Western blotting. Results: Both ursolic acid and gemcitabine could significantly inhibit the proliferation of PANC-1 cells, and the IC50 is 13.67 and 2.78 µmol/L, respectively; and the final concentrations were determined at 2 and 0.2 µmol/L for ursolic acid and gemcitabine, respectively. Compared with single drug treatment, the combined treatment exerted a more prominent cell proliferation inhibition effect ([46.47±5.07]% vs [78.38±8.65]%, [76.12±3.23]%, all P<0.05), apoptosis-induction effect ([39.78± 7.01]% vs [20.35±8.51]%, [20.35±8.51]%, all P<0.01) and migration inhibition effect (P<0.01) on PANC-1 cells. Western blotting showed that the combined treatment strongly inhibited Bcl-2 and IL-6 expression, accelerated P-JNK protein expression compared with single drug treatment. Conclusion: The synergistic effect of ursolic acid and gemcitabine enhanced the inhibition on proliferation, migration, and promoted cell apoptosis of human pancreatic cancer cell line PANC-1, the mechanism may be associated with inhibition of Bcl-2, Il-6, P-stat 3 proteins and promotion of P-JNK protein.
- Full text:20180307.pdf
