Suppressive effect of Tet-on mediated livin RNA interference on growth of lung carcinoma xenegraft in a rat model
10.3872/j.issn.1007-385X.2018.03.004
- VernacularTitle:四环素调控livin RNA干扰系统对肺癌移植瘤生长的影响
- Author:
LI Hongru
1
,
2
;
TU Xunwei
1
;
WENG Lihong
1
;
CHEN Yusheng
1
,
2
Author Information
1. 1. Department of Respiratory and Critical Care Medicine, Fujian Provincial Hospital, Provincial Clinical Medical College, Fujian Medical University, Fuzhou 350001, Fujian, China
2. 2. Research Institute of Four Respiratory Diseases of Fujian Province, Fuzhou 350001, Fujian, China
- Publication Type:Journal Article
- Keywords:
livin gene;
Tet-on mediated regulation;
livin RNAinterfere;
lung carcinoma;
A549 cell line;
animal model
- From:
Chinese Journal of Cancer Biotherapy
2018;25(3):236-239
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate he effect of tetracycline- (Tet-on) mediated livin RNA interference on growth of lung carcinoma xenegrafts, and find a better regulatory way to interfere the development on lung cancer. Methods: livin shRNA lentiviral vectors were constructed; and the lung cancerA549 cells were subcutaneously injected into right upper back of nude mice to establish xenegraft model. The livin shRNAlentiviral vectors were injected into xenografts to interfere the expression of livin, then tetracycline was injected intraperitoneally for the induction. The suppressive effect of Tet-on mediated livin RNA interference efficiency was investigated and lung cancer xenograft development was observed. Results: After the induction with Tet-on, livin gene expression was significantly inhibited by livin shRNAcompared with the control group and Tet-on-NC group; the xenograft volume in Tet-on- livin shRNAgroup was significantly smaller than that in control group and Tet-on-NC group ([5.31±0.86]g vs [8.22±0.63]g and [7.17±0.54] g, P<0.05). Moreover, little body toxicity was observed and no nude mice died in this study. Conclusion: The Tet-on mediated livin shRNA could suppress the growth of lung cancer development with good targeting and controllable characteristics, which might provide a potent tool for treating lung cancer with livin protein as target.
- Full text:20180304.pdf