Photodynamic effects of gold nanostars loading chlorin e6 on lung cancer A549 cells
10.3872/j.issn.1007-385X.2018.04.013
- VernacularTitle:金纳米星负载二氢卟吩e6对肺癌A549细胞的光动力效应
- Author:
LI Chenlu
1
;
XIA Fangfang
2
;
ZHANG Amin
2
;
CUI Daxiang
1
Author Information
1. (1.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325000,Zhejiang,China
2. ； 2.Institute of Nano Biomedicine and Engineering ,Shanghai Jiao Tong University, Shanghai 200240, China
- Publication Type:Journal Article
- Keywords:
gold nanostars;
Chlorin e6;
lung cancer;
A549 cells;
photodynamic therapy
- From:
Chinese Journal of Cancer Biotherapy
2018;25(4):394-400
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To prepare GNS (gold nanostars) loading photosensitizer chlorin e6 (Ce6) and to investigate its photodynamic effects on lung cancerA549 cells. Methods: GNS was firstly modified by SH-PEG-NH2 and then mixed with Ce6 and shaken overnight to prepare GNS-PEG@Ce6, which had photodynamic therapy effects. The characterization, morphology and encapsulation rate were detected. The difference between the phagocytosis of Ce6 and GNS-PEG@Ce6 by A549 cells were observed with a Leical TCS SP8 confocal laser scanning microscope. MTT assay was used to examine the inhibitory effect of GNS-PEG@Ce6 on the proliferation of A549 cells while FCM was used to detect the effect of probe GNS-PEG@Ce6 on the apoptosis ofA549 cells. Results: The particle size of the GNS-PEG@Ce6 was about 100 nm. The prepared GNS-PEG@Ce6 nanoparticles exhibited good dispersion and stability and the encapsulation rate of Ce6 was about 50%. GNS-PEG@Ce6 entered the cells by endocytosis and mainly distributed in the cytoplasm; compared with Ce6, GNS-PEG@Ce6 could enter the cells more effectively. The proliferation-suppression effect of GNS-PEG@Ce6 on A549 cells was significantly stronger than that of Ce6 (P<0.05). The results of flow cytometry showed that the probe exhibited strong apoptotic effect on A549 cells. Conclusion: GNS, as the drug carrier, could effectively increase the Ce6 uptake efficacy in A549 cells, thus further enhancing the killing effects of Ce6 on lung cancerA549 cells.
- Full text:20180413.pdf
