Study of the Cellular Immune Response according to Hemodialysis Duration in Chronic Renal Failure Patients.
- Author:
Dong Eun PARK
1
;
Kyoung Keun LEE
;
Kwon Mook CHAE
;
Joo Heung SONG
;
Heon Taek JEONG
;
Byung Jun SO
Author Information
1. Department of Surgery, School of Medicine, Wonkang University, Iksan, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Cell mediated immunity;
Duration of Hemodialysis;
Chronic renal failure
- MeSH:
Healthy Volunteers;
Humans;
Immunity, Cellular*;
Interleukin-2;
Kidney;
Kidney Failure, Chronic*;
Lymphocytes;
Renal Dialysis*;
T-Lymphocytes;
Uremia
- From:Journal of the Korean Surgical Society
2000;58(1):50-57
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In order to evaluate the effects of the duration of hemodialysis on cell mediated immunity, we studied cellular immune responses in vitro in 12 patients on chronic hemodialysis and in 6 healthy volunteers with normal kidney function. METHODS: The patients on maintenance hemodialysis were divided into two subgroups according to the duration of the hemodialysis: Group 1 (within 5 years, n=6) and Group 2 (>5 yr and < or =10 yr, n=6). Group 3 include the normal control (n=6). The peripheral blood lymphocytes of each group were cultured in RPMI medium 1640 without/or with 3 microgram of phytohemagglutinin (=PHA) for 7 days. RESULTS: CD4 /CD8 ratio at 48hours was totally comparable between the uremic patients (Group 1 and Group 2) and the controls (Group 3). The proportions of CD25 T lymphocytes after 48 hours culture were abnormally high: 7.1+/-0.5% and 7.0+/-1.3% in the uremic patients as compared to 2.5+/-0.6 in the normal controls at the basal state and 66.7+/-2.6% and 68.8+/-1.9% in the uremic patients as compared to 78.3+/-4.6% in the normal controls at the PHA-stimulated condition. The spontaneous production of IL-2 (mean pg SEM) was significantly lower in hemodialized patients (Group 1: 34.5+/-6.0 pg/ml, Group 2: 33.8+/-6.2 pg/ml) as compared to the normal patients (Group 3: 58.8+/-10.4 pg/ml). The PHA stimulated IL-2 production was also significantly reduced in the hemodialized patients (Group 1: 53.2+/- 13.3 pg/ml, Group: 53.0+/-10.3 pg/ml) as compared to the normal patients (Group 3: 150.0+/-24.5 pg/ml). The mean proliferative response to PHA at each point in the hemodialized patients were significantly lower than those of the controls. CONCLUSIONS: These data show that there is no significant correlation between the level of the cell mediated immune response and the duration of hemodialysis. Several defects in the cell mediated immune response associated with uremia might start at an early phase of the hemodialysis.
