Expression of FOXD1 in glioma tissues and its correlation with prognosis of patients
10.3872/j.issn.1007-385X.2018.04.006
- VernacularTitle:FOXD1在胶质瘤组织中的表达及其与患者预后的关系
- Author:
JIANG Zeyu
1
,
2
;
LU Weicheng
3
;
GUO Zongze
1
,
2
;
CHENG Peng
3
Author Information
1. 1. Department of Surgery, Tianjin Children&rsquo
2. s Hospital, Tianjin 300074, China
3. Department of Neurosurgery, First Hospital of China Medical University, Shenyang 110001
- Publication Type:Journal Article
- Keywords:
glioma;
forkhead box（FOX) gene;
survival rate;
prognosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(4):351-356
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To evaluate the expression level of FOXD1 in glioma tissues of different grades, and to investigate the correlation between the expression of FOXD1 and the prognosis of glioma patients. Methods: The tumor tissues were collected from 40 glioma patients, who received surgical treatment in the neurosurgery department of the First Hospital of China Medical University from September 2014 to February 2015; Seven non-tumor tissues obtained from patients underwent internal decompression for traumatic brain injury were used as controls. The FOXD1 expression in glioma and non-tumor brain tissues was analyzed by qRT-PCR and IHC, and the correlations between clinical pathological features of glioma patients and FOXD1 expression level were analyzed. Furthermore, the Kaplan-Meier method was used to analyze the relationship between FOXD1 expression and survival time of patients. In addition, the expression of FOXD1 in glioma tissues and its relationship with patients’prognosis were confirmed by the data from GEO (GSE4290, GSE2223) and Rembrandt database. Results: qRT-PCR showed that the FOXD1 mRNA expression in glioma tissues of WHO grade IV was significantly higher than that of non-tumor brain tissues and glioma tissues of WHO grade II (P<0.01). German immunohistochemical score (GIS) was used to evaluate the immunohistochemical staining intensity, and the relationship between FOXD1 expression and clinical pathological features was analyzed. The results showed that FOXD1 in glioma tissues was related to WHO phathological grade level (χ2=11.73， P<0.01). There was statistically significant difference between the survival time of FOXD1 high expression group and FOXD1 low expression group (P=0.043). The data from GEO data base (GSE4290, GSE2223) and Rembrandt datasets showed that glioma tissues have a higher FOXD1 mRNA expression level than normal brain tissues, and the elevated expression of FOXD1 mRNA was negatively associated with the survival time of glioma patients. Conclusion: FOXD1 was highly expressed in glioma tissues, and the expression level of FOXD1 was increased as the pathological grade increases. The elevated expression of FOXD1 was related with the poor survival of glioma patients.
- Full text:20180406.pdf
