Expression of zinc transporter 1 gene in brain glioma tissues and its effects on proliferation, migration and invasion of glioma U87 cell line
10.3872/j.issn.1007-385X.2018.04.005
- VernacularTitle:锌转运体1基因在脑胶质瘤组织中的表达及其对U87细胞增殖、迁移和 侵袭能力的影响
- Author:
WANG Wei
1
;
ZHANG Luyang
1
;
ZHANG Dongyong
1
;
QIU Bo
1
;
WANG Yunjie
1
;
BAO Yijun
1
Author Information
1. Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China
- Publication Type:Journal Article
- Keywords:
zinc transporter 1 gene;
glioma;
U87 cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(4):346-350
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To detect the expression of zinc transporter 1 (ZnT1) gene in glioma tissue, and to explore its effect on the proliferation, migration and invasion of U87 cells. Methods: From October 2015 to January 2017, 20 patients with glioma, who received no chemoradiotherapy before operation, were collected from Department of Neurosurgery, the First Affiliated Hospital of China Medical University. The protein and mRNA content of ZnT1 in glioma tissues and adjacent tissues were detected by Western blotting and Realtime PCR, respectively. ZnT1 and si-ZnT1 plasmids were transfected into glioma U87 cell line respectively to construct ZnT1 over-expression U87 cell line and ZnT1 knockdown U87 cell line. The effects of ZnT1 on proliferation, migration and invasion of U87 cells were detected by MTT and transwell assay. Results: Both mRNA and protein expressions of ZnT1 in glioma tissues was significantly higher than those in adjacent tissues (all P<0.05). U87 cell lines with ZnT1 over-expression and knockdown were successfully constructed. Compared with the control group and empty plasmid control group, the proliferation (0.54±0.01 vs 0.45±0.04, 0.43±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 over-expression were significantly increased at 12 h after transfection; however, the proliferation (0.37±0.03 vs 0.45±0.01, 0.44±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 knockdown were decreased significantly. Conclusion: ZnT1 was highly expressed in glioma tissues, and promoted the proliferation, migration and invasion of glioma U87 cells.
- Full text:20180405.pdf
