Asiatic acid enhances the chemosensitivity of U87MG glioma cells to paclitaxel through inhibiting the expression of drug resistance related proteins
10.3872/j.issn.1007-385x.2018.04.004
- VernacularTitle:积雪草酸通过抑制耐药相关蛋白表达增强U87MG胶质瘤细胞对紫杉醇 的敏感性
- Author:
ZHANG Lei
1
,
2
,
3
,
4
;
CHEN Lei
2
,
3
,
4
,
5
;
CHEN Jie
1
,
2
,
3
,
4
;
YANG Jingjing
1
,
2
,
3
,
4
Author Information
1. a. Department of Pediatrics, People&rsquo
2. s Hospital of Shizhong District of Ji&rsquo
3. nan City, Ji&rsquo
4. nan 250023, Shandong, China
5. b. Department of Oncology, People&rsquo
- Publication Type:Journal Article
- Keywords:
asiatic acid;
glioma;
drug resistance;
chemosensitivity;
paclitaxel;
U87MG
- From:
Chinese Journal of Cancer Biotherapy
2018;25(4):340-345
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the inhibitive effect of asiatic acid (AA) on paclitaxel (PTX)-resistant glioma cells and its possible mechanism. Methods: The effects of AA on the proliferation and apoptosis of glioblastoma U87MG cells were detected by CCK-8 assay, Real-time quantitative polymerase chain reaction (qPCR) and Western blotting. The drug-resistant glioma cell line PR-U87MG was established by culturing the cells in concentration-increasing PTX. With U87MG cells as control, the PTX-resistance of PR-U87MG cells was confirmed using CCK-8 assay, and the mRNA and protein levels of MDR1 and LRP were measured with qPCR and western blotting. PR-U87MG cells were treated with AA, PTX or AA+PTX, and then the cell viability and apoptosis of each group were measured with CCK-8 assay, qPCR and Western blotting. Results: PTX-resistant PR-U87MG cell line was successfully established. AA inhibited the viability of U87MG and PR-U87MG cells in a dose-dependent manner (P<0.01) and significantly promoted their apoptosis (P<0.01). Compared with the group treated with AA or PTX alone, the group treated with the combination of AA and PTX had significantly decreased protein levels of PARP1 (P<0.01), drug-resistant related proteins (Pgp-1 and LRP [lung resistance protein], all P< 0.01), and markedly increased caspase 3 (P<0.01). Conclusion: AA could effectively enhance the sensitivity of U87MG cells to PTX, and the mechanism may be related to the suppressed expression of drug efflux-associated proteins Pgp-1 and LRP.
- Full text:20180404.pdf
