Effects of siRNA interfering TWSG1 expression on proliferation and apoptosis of gastric cancer MGC-803 cells
10.3872/j.issn.1007-385x.2018.05.007
- VernacularTitle:siRNA干扰TWSG1基因表达对胃癌MGC-803细胞增殖及凋亡的影响
- Author:
ZENG Jiali
1
;
YUAN Jingyi
2
;
SHUAI Chun
3
,
4
;
LIU Yue
Author Information
1. 1a. Grade 2014 Basic Medical Science
2. 1b. Grade 2013 Basic Medical Science
3. 2.Neonatal Department, Women and Children'
4. s Hospital of Guangdong Pro 1c. Department of Biochemistry, 1d. Guangdong Provincial Key Laboratory of Single-Cell Technology and Application, Southern Medical University, Guangzhou 510515, Guangdong, Chinavince, Guangzhou 510010, Guangdong, China
- Publication Type:Journal Article
- Keywords:
TWSG1 gene;
gastric cancer;
MGC-803 cell;
RNAinterference;
proliferation;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(5):480-484
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To study the effects of twisted gastrulation protein homolog 1 (TWSG1) gene on proliferation and apoptosis of gastric cancer MGC-803 cells. Methods: Three siRNAs for TWSG1 gene were designed.The MGC-803 cells at logarithmic phase were divided into blank control group, negative control group (siRNA-NC), siRNA1 interference group, siRNA2 interference group and siRNA3 interference group by transfecting with relevant vectors. The mRNAand protein expressions of TWSG1 in each group were identified by qPCR and Western blotting, respectively; and the stable cell line with highest interference efficiency was screened.The proliferation of cells in each group was detected by CCK-8 assay, and the apoptosis of three groups was detected by flow cytometry. Results: The results of qPCR and Western blotting showed siRNA1 exhibited highest interference efficiency. Compared with the blank control group and the negative control group, the expression of TWSG1 in siRNAinterference cell group was lower (P<0.05), the cell proliferation significantly increased (P<0.05), and apoptosis significantly reduced (P<0.05). Conclusion: siRNA interfering TWSG1 expression in MGC-803 cells can promote cell proliferation, inhibit cell apoptosis.
- Full text:20180507.pdf
