Glucose transporter-1 promotes migration of osteosarcoma MG63 cells via the Wnt/β-catenin pathway
10.3872/j.issn.1007-385x.2018.05.005
- VernacularTitle:葡萄糖转运蛋白-1通过Wnt/β-catenin通路促进骨肉瘤MG63细胞迁移
- Author:
HAO Junlong
1
;
WANG Yapeng
1
;
YANG Kai
1
;
QI Jin
1
;
WANG Xun
1
;
WANG Jing
1
Author Information
1. （Department of Bone Oncology, The Second Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu, China
- Publication Type:Journal Article
- Keywords:
glucose transporter-1(Glut-1);
osteosarcoma;
MG63 cell;
Wnt/β-Catenin pathway;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(5):469-474
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the mechanism of glucose transport protein-1(Glut-1) promoting the migration of osteosarcoma MG63 cells through Wnt/β-catenin pathway. Methods: RNA interference recombinant adenovirus targeting Glut-1 gene (Ad-Glut-siRNA) and control recombinant adenovirus (Ad-GFP) were constructed and transfected into MG63 cells to silence Glut-1 gene expression. The cell migration ability of Blank group, Ad-AFP group, Ad-Glut-siRNA group and AZD2858 (inhibitor of GSK-3) group were detected by Transwell chamber migration assay. Immunofluorescence assay was used to detect the expression of E-cadherin and vimentin in each group and the nuclear translocation of β-catenin. The expression of MMP-2 and MMP-9 in each group and FZD7, β-catenin, Dsh protein in Blank group, Ad-AFP group, Ad-Glut-siRNA group were detected by Western blotting assay. Results: The migration ability of MG63 cells was significantly decreased (P<0.05) after Glut-1 gene silencing, which was restored afterAZD2858 treatment (P <0.05). Compared with Blank group and Ad-GFP group, the E-cadherin level in MG63 cells in Ad-Glut-siRNA group was significantly increased (P<0.05), while the expressions of vimentin, MMP-2, MMP-9, FZD7, β-catenin and Dsh protein were significantly reduced (all P<0.05). Compared with Ad-Glut-siRNA group, E-cadherin expression of AZD2858 group was significantly reduced, while the expressions of vimentin, MMP-2, MMP-9 were significantly up-regulated (P<0.05). Conclusion: The high expression of Glut-1 gene is closely related to the invasion and metastasis of MG63 cells. The possible mechanism is that the high expression of Glut-1 leads to the activation of Wnt/β-catenin pathway, which leads to the decrease of EMT-related protein E-cadherin, and the increase of vimentin and MMP-2, MMP-9, and further promotes the migration of MG63 cells.
- Full text:20180505.pdf
