Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits
10.12016/j.issn.2096-1456.2017.11.002
- Author:
YOU Xiaoqing
1
;
LUO Kai
1
;
LI Yanfen
1
;
WU Chunfang
1
;
YAN Fuhua
2
Author Information
1. he affiliated Stomatological Hospital, Fujian Medical University, Key laboratory of Stomatology, Fujian Province University, Fujian Biological Materials Engineering and Technology Center of Stomatology
2. Nanjing Stomatological Hospital, Medical School of Nanjing University
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis;
Lipopolysaccharide;
Hyperlipidemia;
Peritoneal macrophage;
Inflammation factor;
Periodontitis
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(11):687-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To compare the inflammatory reaction of peritoneal macrophage after Pg-LPS stimulated in healthy rabbit and hyperlipidemia rabbit.
Methods :12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high-fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups: control, 1 μg/mL Pg-LPS, and 1 μg/mL E.coli-LPS. After 24 h treatment, the expressions of C-reaction protein (CRP), interleukin-1β (IL-1β), interleukin-6 (IL-6) , interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were detected by realtime PCR.
Results :The levels of CRP, IL-1β, IL-6, IL-8, and TNF-α were higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg-LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P < 0.05).
Conclusion:Pg-LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.
- Full text:牙龈卟啉单胞菌脂多糖对高脂血症兔腹腔巨噬细胞炎症相关因子基因表达的影响.pdf
