Evaluation of GenoType® MTBDRplus VER2.0 kit for detecting mycobacterium tuberculosis complex in sputum
10.3969/j.issn.1006-2483.2020.02.009
- VernacularTitle:GenoType® MTBDRplus VER2.0试剂盒检测痰标本中结核分枝杆菌复合群的效果评价
- Author:
Cheng MU
1
;
Lina JIANG
1
;
Hui ZHAO
1
;
Zhirui WANG
1
;
Chunhua WANG
1
Author Information
1. Tuberculosis Reference Laboratory, Tianjin Center for Tuberculosis Control，Tianjin 300041，China
- Publication Type:Journal Article
- Keywords:
Tuberculosis;
Molecular line probe assay;
Mycobacterium tuberculosis;
Sputum
- From:
Journal of Public Health and Preventive Medicine
2020;31(2):38-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of GenoType®MTBDRplus VER2.0 kit for detecting Mycobacterium tuberculosis complex (MTBC) in sputum. Methods Sputum samples from 177 patients with suspected tuberculosis were collected, and tested by smear, MGIT liquid culture and GenoType® MTBDRplus VER2.0. When the liquid culture was positive, identification of strains was carried out. The results were statistically analyzed using SPSS 22.0, and the consistency of the count data was compared using the Kappa test. Good consistency was defined as K≥0.75. The sensitivity and specificity were applied to evaluate the performance of GenoType® MTBDRplus VER2.0 kit for detecting MTBC in sputum. Results Based on the MGIT liquid culture results,，the sensitivity, specificity, positive predictive value, negative predictive value and total coincidence rate of the GenoType® MTBDRplus VER2.0 kit was 91.67%, 95.58%, 91.67%, 95.58% and 94.22%，respectively．The Kappa test was performed on both methods, K=0.872 (P<0.001). Conclusion GenoType® MTBDRplus VER2.0 kit had high sensitivity and specificity for detecting MTBC in sputum, and it has good application value for early diagnosis of tuberculosis.
