Effect of alantolactone on malignant biological behaviors of human osteosarcoma 143B cells
10.3872/j.issn.1007-385x.2020.04.006
- VernacularTitle:土木香内酯对人骨肉瘤143B细胞恶性生物学行为的影响
- Author:
YANG Chunmei
1
;
ZHANG Lulu
1
;
HUANG Huakun
1
;
YUAN Xiaohui
1
;
ZHANG Ping
1
;
YE Caihong
1
;
WEI Mengqi
1
;
HUANG Yanran
2
;
LUO Xiaoji
2
;
LUO Jinyong
1
Author Information
1. a. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), School of Laboratory Medicine
2. b. Department of Orthopedics, the FirstAffiliated Hospital of Chongqing Medical University, Chongqing 400016, China
- Publication Type:Journal Article
- Keywords:
alantolactone (ALT);
osteosarcoma;
143B cell;
migration;
invasion;
apoptosis;
Wnt/β-catenin signaling pathway
- From:
Chinese Journal of Cancer Biotherapy
2020;27(4):377-384
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of alantolactone (ALT) on proliferation, migration, invasion and apoptosis of human osteosarcoma 143B cells and the underlying mechanism. Methods: Osteosarcoma 143B cells were treated with different concentrations of ALT (0, 4, 6, 8, 10 µmol/L). Then, the cell proliferation ability was detected by crystal violet staining and MTT assay, cell migration was determined by Wound-healing test, cell invasion was analyzed by Transwell assay and cell apoptosis rate was detected by Hoechst33258 staining. The mRNA and protein expressions of E-cadherin, N-cadherin, caspase-3, cleaved caspase-3 (c-caspase-3), poly ADP-ribose polymerase (PARP) and cleaved PARP (c-PARP) in 143B cells were detected by qPCR and Western blotting (WB), respectively. TCF/LEF (T cell lymphocyte factor/lymphoid enhancer factor) transcriptional activity was examined with Luciferase reporter gene assay. The mRNA and protein expressions of β-catenin as well as MMP-7 and c-Myc were detected by qPCR and WB, respectively. Results: ALT inhibited proliferation, migration and invasion of osteosarcoma143B cells and promoted apoptosis(P<0.05or P<0.01). After the treatment with ALT at 8, 10 µmol/L, the mRNA and protein expressions of E-cadherin and PARP, as well as the protein expressions of c-caspase-3 and c-PARP were up-regulated, while the mRNA and protein expressions of N-cadherin were downregulated (P<0.05 or P<0.01);At the sametime, theTCF/LEF transcriptional activity and the mRNA and protein expressions of β-catenin, MMP-7 and c-Myc were significantly down-regulated (P<0.05 or P<0.01). Conclusion:ALT may inhibit the proliferation, migration and invasion and promote cell apoptosis possibly through suppressing Wnt/β-catenin signaling pathway in osteosarcoma 143B cells.
- Full text:20200406.pdf
