Transformation of malignant biological behaviors and PD-L1 expression in esophageal squamous cell carcinomaYES-2 cell induced by CDDPresistance
10.3872/j.issn.1007-385x.2020.04.004
- VernacularTitle:食管鳞状细胞癌YES-2细胞顺铂耐药致其恶性生物学行为及PD-L1表达 的变化
- Author:
TIAN Cong
1
;
JIA Yunlong
1
;
LYU Wei
1
;
ZHANG Pingmei
1
;
WANG Yu
1
;
LIU Lihua
1
Author Information
1. (Department of Tumor Immunotherapy, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, Hebei, China
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma (ESCC);
YES-2 cell;
YES-2/CDDP-R cell;
cis-dichlorodiammine platinum (CDDP);
chemotherapy resistance;
programmed cell death-ligand 1 (PD-L1)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(4):365-369
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the changes in malignant biological behaviors and expression of programmed cell death-ligand 1 (PD-L1) in esophageal squamous cell carcinoma (ESCC) YES-2 cell line after cis-dichlorodiammine platinum (CDDP) induction (YES-2/CDDP-R). Methods: YES-2 cells were treated with CDDP from low concentration to high concentration (0.25-2.0 μg/ml) with intermittent impact (15-25 days per concentration) to establish ESCC CDDP-resistant cell line YES-2/CDDP-R. The morphological change of YES-2/CDDP-R cells was observed under the inverted microscope. Methyl thiazolyl tetrazolium (MTT) was used to detect cell sensitivity to CDDP. Wound healing assay was used to detect cell migration ability. qPCR and Western blotting were used to detect mRNA and protein expressions of PD-L1. Results: After CDDP gradien ttreatment for9 months,YES-2/CDDP-R cells were successfully established. The morphology of the YES-2/CDDP-R cells showed uneven size, intracellular vacuoles and significantly increased black particles along with the appearance of huge cells. The IC50 of CDDP for YES-2/CDDP-R cells was significantly higher than that for parental cells, indicating decreased sensitivity to CDDP (P<0.05). Compared to theYES-2 cells, the proliferation and migration of YES-2/CDDP-R cells were significantly increased (P<0.05 or P<0.01), and the mRNA and protein expressions of PD-L1 were significantly up-regulated (all P<0.001). Conclusion: YES-2 cells with CDDP resistance (YES-2/CDDP-R) were successfully established. The sensitivity of YES-2/CDDP-R cells to CDDP was significantly reduced while the abilities of cell proliferation and migration were enhanced. The up-regulation of PD-L1 in YES-2/CDDP-R cells suggests that CDDP-resistance could promote immune escape by inducing PD-L1 up-regulation.
- Full text:20200404.pdf
