Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation
10.11665/j.issn.1000-5048.20200209
- VernacularTitle:雷公藤红素联合用药对HepG2细胞增殖的抑制作用
- Author:
Xue ZHANG
1
;
Huizhi OUYANG
;
Wei XU
;
Xiaoying WANG
Author Information
1. 福建中医药大学药学院
- Publication Type:Journal Article
- Keywords:
celastrol;
rhein;
paclitaxel;
glycyrrhetic acid;
HepG2 cells;
combined medication;
synergy effect
- From:
Journal of China Pharmaceutical University
2020;51(2):185-192
- CountryChina
- Language:Chinese
-
Abstract:
To study the inhibitory effect of celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein on the proliferation of human hepatoma cell line HepG2. The MTT method was used to detect the survival rate of HepG2 cells. The cooperativity index(CI)and Jin′s formula method were used to determine the synergistic effect. Apoptosis and cell cycle arrest were detected, too. The results show that celastrol, glycyrrhetinic acid, rhein, and paclitaxel alone can inhibit the proliferation of HepG2 cells, respectively. Combination with glycyrrhetic acid, paclitaxel, and rhein, respectively, the inhibitory effect of celastrol on the proliferation of HepG2 cells was significantly enhanced. And the synergistic effect on the proliferation inhibition of HepG2 cells in some concentrations was displayed in the experiment. The cell apoptosis rate was improved(P< 0. 01)and more cells were arrested in G2/M phase. Celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein displayed a synergistic inhibitory effect on the proliferation of HepG2 cells, and the effect was related to inducing cell apoptosis and increasing the cell cycle arrest in G2/M phase.
