Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases
10.11665/j.issn.1000-5048.20200208
- VernacularTitle:利多卡因抑制基质金属蛋白酶活性改善脂多糖诱导的小鼠急性肺损伤的机制研究
- Author:
Binbin ZHENG
1
;
Jianan ZHANG
;
Yixin FAN
;
Liang HU
;
Wentao LIU
;
Xuerong WANG
Author Information
1. 南京医科大学基础医学院
- Publication Type:Journal Article
- Keywords:
lidocaine;
lipopolysaccharide;
acute lung injury;
matrix metalloproteinases
- From:
Journal of China Pharmaceutical University
2020;51(2):180-184
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the effects and mechanisms of lidocaine on lipopolysaccharide(LPS)-induced matrix metalloproteinase(MMP)-9 and MMP-2 activity in plasma, and the effects of lidocaine on LPS-induced acute lung injury(ALI). Mice were pretreated with lidocaine(2, 4, 8 mg/kg )for 30 minutes, and then treated with 10 mg/kg LPS(ip)for 12 h to induce ALI. The 7-day survival rate and lung wet/dry weight ratio of mice were monitored. Phosphorylation level of p38 was measured by western blot. The activity of MMP-9 and MMP-2 in plasma was evaluated by gelatin zymography. The results showed that pretreatment with lidocaine could significantly reduce the death rate of ALI mice as well as the lung wet/dry weight ratio in a dose-dependent manner and suppress the activity of MMP-9 and MMP-2 in plasma. Moreover, lidocaine also markedly inhibited LPS-induced upregulation of p-p38 in a dose-dependent manner. In conclusion, lidocaine alleviated LPS-induced acute lung injury by suppressing MMP-9 and MMP-2 activity.
