HPLC dual-wavelength fingerprint of Artemisia rupestris L.
10.11665/j.issn.1000-5048.20200207
- VernacularTitle:新疆一枝蒿药材双波长HPLC指纹图谱的研究
- Author:
Yifan ZHANG
1
;
Fengxia BAO
;
Zhenjiang XIA
;
Jing SHANG
Author Information
1. 中国药科大学中药学院
- Publication Type:Journal Article
- Keywords:
Artemisia rupestris L.;
HPLC;
dual-wavelength;
fingerprint;
quality control
- From:
Journal of China Pharmaceutical University
2020;51(2):175-179
- CountryChina
- Language:Chinese
-
Abstract:
To establish HPLC dual-wavelength fingerprint of Artemisia rupestris L. , and provide a scientific basis for the improvement of its quality specifications. The separation was performed on an Agilent Zorbax SB-C18(4. 6 mm×250 mm, 5 μm)column maintained at 32 ℃, with methanol-0. 2% formic acid-water gradient elution at flow rate of 1. 0 mL/min and the UV detection wavelength at both 245 and 325 nm. The sample injection volume was 20 μL. The fingerprint of Artemisia rupestris L. was established. 7 and 8 common peaks were found, respectively, of which 5 common peaks were identified, and the similarity among 9 batches of Artemisia rupestris L. and the fingerprints of control was over 0. 9. The HPLC dual-wavelength fingerprint of Artemisia rupestris L. was established for the first time, providing a new scientific basis for its identification and quality control.
