Licorice enhances radio-sensitivity of nasopharyngeal carcinoma CNE-2 cells by affecting autophagy
10.3872/j.issn.1007-385x.2018.06.009
- VernacularTitle:甘草素通过影响鼻咽癌CNE-2细胞的自噬增强其放疗敏感性
- Author:
ZHANG Rongfang
1
;
WANG Chunling
1
;
LI Shen
1
;
JIN Haike1
1
;
JI Yinghua
2
Author Information
1. Henan Nursing Career Academy, Anyang 455000, Henan, China
2. FirstAffiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan, China
- Publication Type:Journal Article
- Keywords:
licorice;
nasopharyngeal carcinoma;
CNE-2-RR cell;
autophagy;
radio-sensitivity
- From:
Chinese Journal of Cancer Biotherapy
2018;25(6):601-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of licorice on radio-sensitization of nasopharyngeal carcinoma CNE-2 cells and its mechanism. Methods: The radio-resistant nasopharyngeal carcinoma cell line (CNE-2-RR) was constructed and cultured in vitro. MTT assay was used to detect the effect of different concentrations of licorice on the proliferation activity of nasopharyngeal carcinoma cells. The changes of autophagosome in CNE-2-RR cells after licorice treatment were observed by transmission electron microscopy (TEM). Western blotting was used to detect the effect of licorice on the level of autophagy protein in CNE-2-RR cells. Single cell gel electrophoresis (comet assay) was used to detect the DNAdamage and repair of different groups of CNE-2-RR cells. Flow cytometry was used to detect the apoptosis rate of CNE-2-RR cell line. Results: Low-radiation resistant CNE-2-RR cell line was successfully constructed; MTT assay showed that 20 mmol/L licorice exhibited highest inhibition on CNE-2-RR cells (58.86 ± 5.02)%. Transmission electron microscopy showed increased autophagicbody and abnormal mitochondria and nuclei morphology in CNE-2-RR cells after treatment. Western blotting showed that autophagic protein LC3-II level was increased and LC3-I level was decreased in CNE-2-RR cells (P < 0.05). The results of single cell gel electrophoresis showed that the length of comet tail distance of CNE-2-RR cells after licorice treatment was higher than that of the control group (P<0.05), indicating weakened repair ability of DNA damage. Conclusion: Licorice enhances the radio-sensitivity of CNE-2-RR cells by influencing autophagy and DNArepair ability.
- Full text:20180609.pdf
