Ginsenoside Rg3 regulates CaM gene expression through PI3K/AKT signal system to promote apoptosis of gastric cancer BGC-823 cell
10.3872/j.issn.1007-385x.2018.06.007
- VernacularTitle:人参皂苷Rg3通过PI3K/AKT信号系统调控CaM基因表达促进胃癌 BGC-823细胞的凋亡
- Author:
SHI Yanyan
1
;
LI Shucai
1
;
SUN Jun
1
Author Information
1. Central Hospital of Jinzhou City, Jinzhou 121001, Liaoning, China
- Publication Type:Journal Article
- Keywords:
ginsenoside Rg3;
calmodulin;
CaM gene;
gastric cancer;
BGC-823 cell;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(6):590-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate Ginsenoside Rg3 interfering the expression of CaM through PI3K/AKT signaling pathway to affect the biological activity of gastric cancer BGC-823 cells. Methods: After the culture and passage of gastric cancer BGC-823 cells, Western blotting was used to detect the expression of p-AKT and CaM protein in gastric cancer BGC-823 cells treated with IGF-1 and/ or Rg3; The effect of IGF-1 and/or Rg3 on the proliferation of BGC-823 cells was detected by MTT assay; The effect of IGF-1 and/or Rg3 on the invasion of BGC-823 cells was detected by Transwell assay; Effect of IGF-1 and/or Rg3 on apoptosis of BGC-823 cells was detected by Flow Cytometry. Results: Western blotting results showed that the expression of p-AKT and CaM protein increased in BGC-823 cells with the prolongation of IGF-1 treatment (all P<0.05); Compared with the blank control group, Rg3 significantly inhibited the proliferation of BGC-823 cells, while IGF-1 and IGF-1+Rg3 significantly promoted the cell proliferation (all P<0.05); Compared with the blank control group, Rg3 significantly reduced the invasion of BGC-823 cells, while IGF-1 and IGF-1+Rg3 significantly promoted the invasion of BGC-823 cells (all P<0.05);Flow cytometry showed that compared with the blank control group, Rg3 significantly promoted the apoptosis of BGC-823 cells, while IGF-1 and IGF-1+Rg3 significantly inhibited the apoptosis of BGC-823 cells (all P< 0.05). Conclusion: Ginsenoside Rg3 inhibits the expression of CaM by blocking PI3K/AKT signaling pathway, thereby promoting the apoptosis of gastric cancer BGC-823 cells.
- Full text:20180607.pdf
