Effect of co-culture with amniotic epithelial cells on biological characteristics of amniotic mesenchymal stem cells
10.3872/j.issn.1007-385x.2018.06.005
- VernacularTitle:与羊膜上皮细胞共培养对羊膜间充质干细胞生物学特性的影响
- Author:
RAN Lijing
1
,
2
;
ZENG Yun
3
;
WANG Shaochun
4
;
ZHANG Disi
3
;
YI Xiangwei
5
,
6
;
HONG Min
3
;
LI Shaoyou
3
;
DONG Jian
3
;
DU Minxia
3
;
SHI Mingxia
1
Author Information
1. 1a. Department of Hematology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China
2. 2. Department of Ultrasonography, the Affiliated Hospital of Jining Medical College, Jining 272000, Shandong, China
3. 1a. Department of Hematology, 1b. Department of Oncology, 1c. Department of Obstetrics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China
4. Department of Ultrasonography, the Affiliated Hospital of Jining Medical College, Jining 272000, Shandong, China
5. 1a. Department of Hematology, 1b. Department of Oncology, 1c. Department of Obstetrics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China, Department of Hematology, People'
6. s Hospital of Xishuangbanna, Dai Autonomous Prefecture, Xishuangbanna 666100,Yunnan, China
- Publication Type:Journal Article
- Keywords:
amniotic mesenchymal stem cell (AMSC);
amniotic epithelial cell (AEC);
CXCR4;
homing;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(6):574-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of this study was to investigate the effect of co-culture with AEC (amniotic epithelial cell) on the biological characteristics of AMSC (amniotic mesenchymal stem cell), and to investigate the roles of SDF-1/CXCR4 axis in the homing and migration of AMSC. Methods: AMSC andAEC were isolated from human amnion, and then cultured, amplified and identified, respectively. TheAMSC were divided into three groups:AEC co-cultured group, serum-free cultured group and serum cultured group.After culture for 24 h, 48 h, and 72 h, the proliferation viability ofAMSC was measured by CCK-8 assay and trypan blue staining; the expression of CXCR4 mRNAwas analyzed by flow cytometry and Real-time RT-PCR, and the migration ability ofAMSC in vitro was observed by migration assay. Results: Cell viability (48 h and 72 h) and survival rate in the co-culture and serum groups were higher than those in the serum-free group (all P<0.05). The mRNA and protein expressions of CXCR4 in AMSC of the co-culture and serum-free groups were significantly higher than those of the serum group (P<0.05). The migration ability of AMSC in the co-culture and serumfree groups, which increase with the SDF-1 (stromal cell derived factor-1) concentration gradient, were higher than that in the serum group (P<0.05). Conclusion: AMSC co-cultured with AEC still have the basic biological characteristics of MSC, and showed good growth activity. Co-culture withAEC can up-regulate CXCR4 onAMSC surfaces and enhance the migration ability ofAMSC in vitro.
- Full text:20180605.pdf
