Effect of shRNA interfering BAMBI on malignant biological behaviors of human colon cancer SW480 cell
10.3872/j.issn.1007-385x.2018.06.004
- VernacularTitle:shRNA干扰BAMBI基因对人结肠癌SW480细胞恶性生物学行为的影响
- Author:
MA Huiwen
1
,
2
,
3
;
WANG Sixiong
1
,
2
,
3
;
WANG Ting
1
,
2
,
3
;
XIAO Xiaoyi
1
,
2
,
3
;
TANG Long
1
,
2
,
3
;
WANG Chunmei
1
,
2
,
3
;
WANG Enwen
1
,
2
,
3
Author Information
1. (Chongqing University Cancer Hospital &
2. Chongqing Cancer Hospital &
3. Chongqing Cancer Institue, Chongqing 400030, China)
- Publication Type:Journal Article
- Keywords:
colon cancer;
bone morphogenetic protein and activin membrane bound inhibitor(BAMBI);
SW480 cell;
malignant biological behavior
- From:
Chinese Journal of Cancer Biotherapy
2018;25(6):568-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of shRNA interfering BAMBI (bone morphogenetic protein and activin membrane bound inhibitor) on the proliferation, apoptosis, invasion and migration of human colon cancer SW480 cells and the possible mechanisms. Methods: After successful transfection with sh-BAMBI in SW480 cells, the mRNA and protein epxressions of BAMBI were detected by qRT-PCR and Western blotting, respectively. Cell proliferation was measured by MTT; apoptosis was tested by Hoechst33258 staining; cell invasion was detected by transwell assay; and cell migration was measured by wound healing assay. The expressions of TGF-β/ Smad/2 signaling pathway related proteins were detected by Western blotting. Results: The mRNA and protein levels of BAMBI in shBAMBI group were lower than those of control group (P<0.05). Compared with control group, cell proliferation in sh-BAMBI group was obviously decreased (P<0.05), while apoptosis was obviously increased (P<0.01); in the meanwhile, cell invasion and migration in sh-BAMBI group were significantly reduced (P<0.05). In addition, the protein level of TGF-β and the ratio of p-Smad/2/ Smad/2 in shBAMBI group were significantly higher than those in control group (P<0.05). Conclusion: Interference of BAMBI by shRNA inhibits proliferation, invasion and migration but induces apoptosis of human colon cancer SW480 cells and activates TGF-β/Smad/2 pathway.
- Full text:20180604.pdf
