Expression of circular RNA ciRS-7 in tissues of sophageal squamous cell carcinoma and its effect on cell biological characteristics of TE1 cells
10.3872/j.issn.1007-385x.2018.07.012
- VernacularTitle:ciRS-7在食管癌组织中的表达及其对食管癌细胞TE1生物学特性的影响
- Author:
MENG Lingjiao
;
DING Pingan
;
JU Yingchao
;
LIU Fei
;
LIU Shina
;
LIU Sihua
;
CHANG Sheng
;
GU Lina
;
SANG Meixiang
- Publication Type:Journal Article
- Keywords:
ciRS-7；esophageal squamous cell cancer(ESCC)；TE1 cell;
proliferation；migration；invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(7):726-732
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of ciRS-7 in esophageal squamous cell carcinoma (ESCC) and its effect on the cellular proliferation, migration and invasion. Methods: The cancer tissues and paired adjacent normal tissues from 60 ESCC patients treated in the Fourth Hospital of Hebei Medical University between May, 2016 andApril, 2017 were selected for this study. The expressions of ciRS-7 were detected by qRT-PCR. After over-expressing or silencing of ciRS-7, the proliferation of ESCC cell line TE1 was measured by CCK-8 assay; and the migration and invasion were tested by wound healing assay and Transwell invasion assay，respectively. Finally, the effect was validated via animal experiment. Results: CiRS-7 was highly expressed in ESCC tissues (P<0.05), and its expression level was closely related to pathological grade and lymph node metastasis (P<0.05). Over-expression of ciRS-7 significantly increased the proliferation, migration and invasion (all P<0.05) of TE1 cells; while silencing of ciRS-7 remarkably suppressed the proliferation, migration and invasion (all P<0.05). Conclusion: CiRS-7 was up-regulated in ESCC and could enhance ESCC cell proliferation, migration and invasion, suggesting that ciRS-7 could be used as a potential target for the diagnosis and treatment of ESCC.
- Full text:20180712.pdf
