miR-1180-5p inhibits proliferation, migration and invasion of prostate cancer cells by activating CDKN1Agene expression
10.3872/j.issn.1007-385x.2018.07.007
- VernacularTitle:miR-1180-5p通过激活CDKN1A基因表达抑制前列腺癌细胞增殖、迁移 和侵袭
- Author:
WANG Yong
1
;
GUO Yonglian
1
;
CHEN Lin
1
;
LI Guohao
1
;
Ying Chengcheng
1
;
CHENG Wei
2
Author Information
1. Department of Urology, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430014, Hubei, China
2. b. Department of Otolaryngology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430077, Hubei, China
- Publication Type:Journal Article
- Keywords:
miR-1180-5p;
CDKN1A gene;
prostate cancer;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(7):698-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of microRNA-1180-5p (miR-1180-5p) on malignant biological behaviors of prostate cancer VCAP and LNCaP cells and the possible mechanisms. Methods: dsControl (dsControl group) and miR-1180-5p (miR-1180-5p group) were constructed and then transfected into two prostate cancer cell lines VCAPand LNCaP. qPCR and Western blotting were used to analyze the changes in mRNA and protein expressions of CDKN1A, Cyclin D1 and CDK6 after transfection. Cell cycle distribution, proliferation activity, clone formation capacity, cell migration and invasion ability were detected by flow cytometry, MTT assay, colony culture assay and Transwell assay, respectively. Results: qPCR results showed that compared with dsControl, CDKN1A mRNA levels in VCAP and LNCaP cells transfected with miR-1180-5p were up-regulated significantly, while the mRNA expressions of Cyclin D1 and CDK6 were significantly down-regulated (all P<0.01). Western blotting result was consistent with that of qPCR. The percentage of cells in G0/G1 phase was increased after transfection of miR-1180-5p (P<0.05), but the proportion of cells in S phase and G2/M phase was decreased and the cell cycle was arrested at G0/G1 phase (P<0.05). The proliferation activity of the two prostate cancer cells was significantly lower than that of the dsControl group after miR-1180-5p transfection (P<0.05), and the number of colonies in the miR-1180-5p group was significantly lower than that in the dsControl group (P<0.01). In the meanwhile, the cell migration and invasion ability in miR-1180-5p group was decreased (P<0.01). Conclusion: miR-1180-5p can significantly activate CDKN1A gene expression in prostate cancer cells and further inhibit the proliferation, migration and invasion of prostate cancer cells.
- Full text:20180707.pdf
