Inhibition of miR-802 on insulin secretion by targeting Hnf1B
10.11665/j.issn.1000-5048.20200115
- VernacularTitle:miR-802靶向Hnf1B抑制胰岛素分泌的作用研究
- Author:
Danwei WANG
1
;
Fangfang ZHANG
;
Liang JIN
;
Jie WU
Author Information
1. 中国药科大学生命科学与技术学院
- Publication Type:Journal Article
- Keywords:
miR-802;
insulin secretion;
Hnf1B;
target gene
- From:
Journal of China Pharmaceutical University
2020;51(1):99-106
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of miR-802 on insulin secretion by islet β cells and its mechanism, miR-802 was overexpressed or knocked down in primary islet cells and Min6 cells via transfecting miR-802 mimic and miR-802 inhibitor, respectively. The effect of miR-802 on insulin secretion was detected by ELISA. The target gene of miR-802 was confirmed by miRNA target gene database prediction, luciferase report and Western blot. The function recovery experiment was carried out to clarify the mechanism of miR-802 regulating β cell secretion of insulin. The results showed that overexpression of miR-802 in islet primary cells and Min6 cells inhibited insulin secretion. qPCR and Western blot showed that miR-802 inhibited insulin secretion by inhibiting the transcription and translation of the target gene, hepatocyte nuclear factor 1β(Hnf1B).
