Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells
10.11665/j.issn.1000-5048.20200106
- VernacularTitle:载紫杉醇的大黄酸偶联物胶束对MCF-7细胞的毒性与细胞摄取研究
- Author:
Xiaoying WANG
1
;
Xiaying WANG
;
Liangzhen QIU
;
Huizhi OUYANG
;
Wei XU
Author Information
1. 福建中医药大学药学院
- Publication Type:Journal Article
- Keywords:
paclitaxel;
carboxymethyl chitosan-rhein conjugate;
polymeric micelles;
anti-tumor;
cellular uptake
- From:
Journal of China Pharmaceutical University
2020;51(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
In this study, in vitro cytotoxicity of carboxymethyl chitosan-rhein conjugate(CR conjugate)and paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles(PTX/CR PMs)was evaluated by MTT method in MCF-7 cells. The results showed that CR conjugate displayed good security; PTX/CR PMs in 24 h showed better antitumor activity than Taxol® . Environment-responsive fluorescent probe P4 was used to determine the cellular uptake of PTX/CR PMs in MCF-7 cells. The results also showed that P4 and PTX co-loaded carboxymethyl chitosan-rhein polymeric micelles ［(P4+PTX)/CR PMs］ could be taken up by MCF-7 cells. There was no difference between(P4+PTX)/CR PMs group and(P4+PTX)/CR PMs with verapamil group, suggesting that CR PMs could protect fluorescent probe and/or drugs in their cores avoiding efflux by P-glycoprotein. These results will contribute to in vivo study of CR conjugate and PTX/CR PMs in the future.
