SNHG6 promotes invasion and metastasis of esophageal squamous cell carcinoma TE1 cells via upregulating ZEB1
10.3872/j.issn.1007-385x.2020.03.011
- VernacularTitle:SNHG6通过上调ZEB1表达促进食管鳞状细胞癌TE1细胞的侵袭和转移
- Author:
WANG Mengjie
1
;
LIU Yan
1
;
LYU Wei
1
;
TIAN Cong
1
;
WANG Yu
1
;
ZHAO Lianmei
2
;
LIU Lihua
1
Author Information
1. a. Department of Tumor Immunotherapy
2. b. Research Center, Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
small nucleolar RNA host gene 6 (SNHG6);
zinc finger E-box binding homeobox factor 1 (ZEB1);
invasion;
metastasis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(3):282-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the roles and mechanisms of long non-coding RNA (lncRNA) small nucleolar RNA host gene 6 (SNHG6) in promoting invasion and metastasis of esophageal squamous carcinoma (ESCC). Methods: Real time quantitative polymerase chain reaction (qPCR) was used to detect the expression of SNHG6 in ESCC and matched para-carcinoma tissues. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the expression of SNHG6 in ESCC cell lines (TE1, Yes-2, Eca9706 and Kyse150). Then, TE1 cell line which harbored highest expression of SNHG6 was used in following experiments. siRNAs were used to knock down the expression of SNHG6. Clone formation, wound-healing and transwell assay were used to detect the abilities of proliferation, migration andinvasionofTE1cells,respectively.Westernblottingwasusedtodetecttheexpressions of MMP-2, MMP-9andZEB1 protein before and after knockdownofSNHG6inTE1cells.Results:SNHG6washighlyexpressedinESCC tissues, compared to para-carcinoma tissues (P<0.01). The expression of SNHG6 was significantly decreased after transfection of SNHG6siRNA (all P<0.01). The abilities of proliferation, migration and invasion of TE1 cells in si-SNHG6-1 and si-SNHG6-2 group were significantly lower than those in the control group (all P<0.01). The expressions of ZEB1, MMP-2and MMP-9 in si-SNHG6-1 and si-SNHG6-2 group were significantly lower than those in the control group (all P<0.05). Conclusion: SNHG6 is highly expressed in ESCC tissues and promotes the malignant biological behavior of ESCC cells. Its mechanism of promoting the occurrence and development of ESCC may be related to the upregulation of ZEB1 expression.
- Full text:20200311.pdf
