miR-377-5p inhibits proliferation and invasion of hepatocellular carcinoma HepG2 cells by down-regulating HIF-1α and related VEGFsignaling pathway
10.3872/j.issn.1007-385x.2020.03.006
- VernacularTitle:miR-377-5p通过下调HIF-1α及其相关VEGF信号通路抑制肝细胞癌 HepG2细胞的增殖和侵袭
- Author:
YANG Jin
1
;
HE Kai
1
;
ZHANG Mengyu
1
;
WU Lile
1
;
QIN Shu
1
;
FENG Chunhong
1
;
LUO Ming
1
;
XIA Xianming
1
Author Information
1. (Department of Hepatobiliary Surgery, theAffiliated Hospital of Southwest Medical University
- Publication Type:Journal Article
- Keywords:
miR-377-5p;
hypoxia inducible factor-1 (HIF-1α);
hepatocellular carcinoma (HCC);
HepG2 cell;
proliferation;
invasion;
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(3):248-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To explore the targeting relationship between miR-377-5p and hypoxia inducible factor-1 (HIF-1α), and investigate the regulatory effect of miR-377-5p on proliferation, invasion and epithelial-mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells through vascular endothelial growth factor (VEGF) signaling pathway. Methods： ：The expression of miR-377-5p in 35 pairs of human HCC tissues and para-cancerous tissues was detected by qPCR. Then, HepG2 cells were divided into control group, mimic-NC group and miR-377-5pmimicgroup.qPCRwasusedto detect the transfection efficiency; the effects of miR-377-5p over-expression on proliferation and invasion of HepG2 cells were examined by EdU staining and Transwell assay, respectively; and the effect of miR-377-5p over-expression on the expressions of proliferation-related protein Ki-67, proliferating cell nuclear antigen (PCNA) and epithelial-mesenchymal transition (EMT) markers (E-cadherin and N-cadherin) were detected by Western blotting (WB); the effect of miR-377-5p over-expression on the expression of hypoxia inducible factor-1α (HIF-1α) in HepG2 cells was detected by qPCR and WB; and the targeting relationship between miR-377-5p and HIF-1α gene was determined by Luciferase reporter gene assay. Results: The expression of miR-377-5p in HCC tissues was significantly lower than that in para-cancerous tissues (P<0.01). Compared with the control group, the expression of miR-377-5p in HepG2 cells of miR-377-5p mimic group elevated significantly, and the proliferation, invasion and the expression of N-caderin proteins decreased,significantly (all P<0.01), while the expression of E-caderin increased significantly (P<0.01). At the same time, the mRNA and protein expressions of HIF-1α in miR-377-5p mimic group decreased significantly (P<0.01 or P<0.05). miR-377-5p targetedly inhibited the expression of HIF-1α gene and suppressed the activation of VEGF pathway (all P<0.05). Conclusion: miR-377-5p inhibits the proliferation, invasion and EMT of HepG2 cells via targetedly inhibiting HIF-1α expression and suppressing the activation of VEGF signaling pathway.
- Full text:20200306.pdf
