Baohuoside- Ⅰ inhibits malignant phenotype of SW480 and RKO cells and its mechanism
10.3872/j.issn.1007-385x.2020.03.003
- VernacularTitle:香加皮宝藿苷-Ⅰ抑制结肠癌细胞株SW480和RKO的恶性表型及其作用 机制
- Author:
WANG Xiaohua
1
;
LI Xiaoya
2
;
BAI Hanyu
2
;
HUO Bingjie
3
Author Information
1. Biology Laboratory, Medical College, Hebei University of Engineering
2. Clinical Laboratory Medicine, Hebei Medical University
3. Department of Traditional Chinese Medicine
- Publication Type:Journal Article
- From:
Chinese Journal of Cancer Biotherapy
2020;27(3):228-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Baohuoside-Ⅰ on the proliferation, invasion, migration and apoptosis of colon cancer cell lines SW480 and RKO and the relative mechanism. Methods: Colon cancer cell lines SW480 and RKO were respectively treated with different concentrations of Baohuoside-Ⅰ (0, 5, 10, 20 μg/ml). Cell proliferation was detected by MTT assay; The ability of cell clone formation was tested by cell clone formation experiments; The migration and invasion of cells were detected by Transwell assay; The apoptosis and cell cycle was detected by Flow cytometry; and the protein expression levels of cleaved PARP, cleaved Caspase-3 and Bcl-2 were detected by Western blotting. TheeffectsofBaohuoside-Ⅰontranscriptomeandpossiblesignaling pathways were detected by RNA-Seq technology. Results: Baohuoside-Ⅰ could inhibit the proliferation, invasion and migration of SW480 and RKO cells, and induce cell apoptosis and G0/G1 phase block. Baohuoside-Ⅰ could also up-regulate the protein expressions of cleaved PARP and cleaved Caspase-3 but down-regulate the protein expression of Bcl-2 in SW480 and RKO cell lines. In addition, RNA-Seq data analysis showed that DNAreplication and transcription of ERBB signaling pathway related genes were both affected by Baohuoside-Ⅰ. Conclusion: Baohuoside-Ⅰ could induce apoptosis and G0/G1 phase block of colon cancer cell lines SW480 and RKO by affecting the expression of apoptosis related proteins, as well as cellular DNA replication and ERBB signaling pathways, thus inhibiting the malignant phenotypes of SW480 and RKO.
- Full text:20200303.pdf
