Value of GeneXpertMTB/RIF and Hain techniques for the diagnosis of sputum smear negative tuberculosis and for the assessment of rifampin resistance
10.3969/j.issn.1006-2483.2020.01.020
- VernacularTitle:GeneXpertMTB/RIF和Hain技术对痰涂片阴性肺结核诊断价值及利福平耐药性的评估
- Author:
Xin ZHOU
1
;
Chunjie TANG
2
Author Information
1. Department of Clinical Laboratory, Shanghai Pudong New Area Pulmonary Hospital, Shanghai 201209, China
2. Administrative Office of Shanghai Pudong New Area Pulmonary Hospital, Shanghai 201209, China
- Publication Type:Journal Article
- Keywords:
GeneXpertMTB/RIF;
Hain;
Sputum smear negative patients;
Pulmonary tuberculosis;
Rifampicin resistance;
Evaluation value
- From:
Journal of Public Health and Preventive Medicine
2020;31(1):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application value of Xpert mycobacterium tuberculosis/rifampicin (GeneXpertMTB/RIF) and Hain techniques for the diagnosis of sputum smear negative tuberculosis and for the assessment of rifampin resistance. Methods A total of 254 patients with suspected pulmonary tuberculosis treated in our hospital from March 2018 to June 2019 were enrolled. GeneXpertMTB/RIF, Hain techniques, Roche culture and drug susceptibility testing were performed. Taking Roche culture results as a gold standard, the efficacy of GeneXpertMTB/RIF and Hain techniques in detecting sputum smear-negative pulmonary tuberculosis was analyzed by ROC curve. Based on the results of drug susceptibility testing, the efficacy of GeneXpertMTB/RIF and Hain techniques in detecting rifampicin resistance was analyzed. Results The sensitivity, specificity and accuracy of GeneXpertMTB/RIF in the diagnosis of sputum smear negative pulmonary tuberculosis were 82.18%, 86.93% and 83.52%, respectively. The sensitivity, specificity and accuracy of Hain technique in the diagnosis of sputum smear negative pulmonary tuberculosis were 67.33%, 77.12% and 73.23%, respectively. The sensitivity, specificity and accuracy of GeneXpertMTB/RIF in evaluating rifampicin resistance were 73.33%, 98.84% and 95.05%, respectively. The sensitivity, specificity and accuracy of Hain technique in evaluating rifampicin resistance were 86.67%, 82.56% and 83.17%, respectively. Conclusion Compared with Hain technology, GeneXpertMTB/RIF had better clinical value for diagnosis of sputum smear negative tuberculosis and for the assessment of rifampin resistance. This study demonstrates that GeneXpertMTB/RIF technique has a good application value in the rapid diagnosis and treatment of sputum smear negative pulmonary tuberculosis.
