Study on Inhibitory Effects of Total Alkaloids of Gelsemium elegans on the Proliferation and Angiogenesis of Human Colon Cancer Cells
- VernacularTitle:钩吻总碱抑制人结肠癌细胞增殖及血管新生的作用研究
- Author:
Wenyi WANG
1
;
Xinghui TAN
1
;
Pingping ZHANG
1
;
Yuke YANG
1
;
Zihong HUANG
1
;
Desen LI
1
;
Shuisheng WU
1
Author Information
1. School of Pharmacy，Fujian University of TCM，Fuzhou 350122，China
- Publication Type:Journal Article
- Keywords:
Total alkaloid of Gelsemium elegans;
Colon cancer;
HT-29 cells;
HUVEC;
Proliferation;
Migration;
Invasion
- From:
China Pharmacy
2020;31(8):957-962
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To investigate the inhibitory effects of total alkaloids of Gelsemium elegans （TAG） on the proliferation and angiogenesis of human colon cancer cells. METHODS ：Human colon cancer cell line HT- 29 and HUVEC were cultured in vitro . After the intervention of low- ，medium-，high-dose TAG （40，80，120 μg/mL），the morphology of the two cells was observed by fluorescence inversion microscope. The survival rate of HT- 29 cells and HUVEC was detected by CCK- 8 assay. Flow cytometry was used to detect HT- 29 cell cycle. The migration rate ，invasion rate and tube number of HUVEC were observed by scratching test ，Transwell invasion experiment and tube formation experiment. RESULTS ：Compared with blank group ，HT-29 cells and HUVEC were decreased to different extents in TAG groups ；dead cells were observed ，and the survival rate of both decreased significantly （P＜0.05 or P＜0.01）. The proportion of HT- 29 cells at G 2/M phase in TAG groups as well as those at G 0/G1 phase in medium-dose group were increased significantly ；the proportion of HT- 29 cells at S phase in TAG groups as well as those at G 0/G1 phase in high-dose group were decreased significantly （P＜0.05 or P＜0.01）. Survival rate ，migration rate and invasion rate of HUVEC were decreased significantly in TAG groups ，and tube number was also decreased significantly at each time point during 4-24 h（P＜0.01）. CONCLUSIONS ：TAG have inhibitory effect on the proliferation of human colon cancer HT- 29 cells and HUVEC，can change HT- 29 cell cycle ，inhibit the migration ，invasion and tube formation of HUVEC.
