Evaluation of the Quality of Taxillus chiueusis from Different Hosts Sources by HPLC Fingerprint Combined with Chemometrics
- VernacularTitle:HPLC特征指纹图谱结合化学计量学评价不同寄主来源的桑寄生药材质量
- Author:
Senhua LU
1
;
Yangying GAN
1
;
Lian TANG
1
;
Fengyi ZHANG
2
;
Benwei SU
3
Author Information
1. Yulin Center for Food and Drug Control，Guangxi Yulin 537000，China
2. Luchuan County Center for Food and Drug Control of Yulin City，Guangxi Yulin 537000，China
3. Qinzhou Institute of Traditional Chinese Medicine，Guangxi Qinzhou 535099，China
- Publication Type:Journal Article
- Keywords:
Taxillus chiueusis;
Different hosts;
HPLC
- From:
China Pharmacy
2020;31(7):794-799
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish a host specific method for the qual ity evaluation of Taxillus chiueusis by evaluating the quality of T. chiueusis from different hosts sources. METHODS ：HPLC was adopted with CAPCELL PAK C 18 MGⅡ column， mobile phase consisted of methanol- 0.2％ phosphoric acid ，volume flow 1.0 mL/min（gradient elution ），detection wavelength of 254 nm，column temperature of 30 ℃. Totally of 16 batches of T. chiueusis from different hosts sources were collected as sample （4 batches from tea host ，maple host ，willow host and mulberry host respectively ）. HPLC characteristic chromatogram was established with TCM Chromatogram Fingerprint Similarity Evaluation System （2004A edition ），and similarity evaluation was performed. The peak was identified by comparison with the reference substance. IBM SPSS 19.0 software was used for cluster analysis and principal component analysis. RESULTS ：Totally 11 common peaks were demarcated ，peak No. 8，10，11 were identified as rutin ，quercetin and quercitin. The similarity of 16 batches of samples was more than 0.9. The chemical components of T. chiueusis from different hosts sources were basically the same （RSDs of relative time of commom peaks were 0.03％-0.40％）， but the contents of the same component were quite different （RSDs of relative peak area of commom peaks were 27.00％-64.20％）. By cluster analysis ，16 batches of T. chiueusis from different hosts sources were divided into 3 types. The results of principle component analysis showed that quercetin ，quercitin and rutin had significant effect on the quality of T. chiueusis ，which could be used as the quality evaluation index ，and the whole quality of maple host was the best （the highest comprehensive score ）. CONCLUSIONS： HPLC fingerprint combined with chemometrics can be used to evaluate the quality of T. chiueusis from different hosts sources.
