Study on lncRNA ADAMTS9-AS2 promoting invasion and metastasis of salivary adenoid cystic carcinoma
10.12016/j.issn.2096-1456.2020.04.002
- Author:
WU Donghui
1
;
ZHU Yunying
1
;
LIANG Jianqiang
1
;
LIN Zhaoyu
2
,
3
;
LI Jinsong
2
,
3
Author Information
1. Department of Oral Surgery, Stomatology hospital of Haizhu district
2. Department of Oral &
3. Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
long noncoding RNA;
lncRNA ADAMTS9-AS2;
salivary adenoid cystic carcinoma;
adenoid cystic carcinoma cell line with high metastasis;
lncRNA expression microarray;
invasion;
metastasis;
RNA interference;
epigenetics
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(4):214-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of lncRNAs in the invasion and metastasis of salivary adenoid cystic carcinoma (SACC) cells.
Methods:With SACC-LM as the experimental group and SACC83 as the control group, lncRNA chips were used to screen the differentially expressed lncRNAs. The differentially expressed lncRNAs were further verified by real-time quantitative RT-PCR (qRT-PCR). The invasion and migration abilities of the adenoid cystic carcinoma cell lines before and after transfection with lncRNA siRNAs were detected by invasion and migration experiments. The clinicopathological features and prognosis of patients with different expression of lncRNAs and SACC were analyzed.
Results:The microarray showed that ADAMTS9-AS2 was highly expressed in the SACC-LM cells. Real-time quantitative RT-PCR further confirmed that ADAMTS9-AS2 was significantly upregulated in the SACC-LM cells. Invasion and migration experiments showed that the invasion and migration were significantly reduced after the expression level of ADAMTS9-AS2 was downregulated (P ＜ 0.001). Analysis of the clinicopathological data showed that ADAMTS9-AS2 was highly expressed in SACC. High expression of ADAMTS9-AS2 was associated with poor prognosis and a high tumor metastasis rate in SACC patients.
Conclusion :High expression of ADAMTS9-AS2 promotes the migration and invasion of SACC cells. ADAMTS9-AS2 is upregulated in the SACC tissues and is related to a high metastasis rate and poor prognosis.
- Full text:长非编码RNA lncRNA ADAMTS9-AS2促进唾液腺腺样囊性癌侵袭转移的研究.pdf
