Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers.
- Author:
Yang HUANG
1
;
Jinsong KONG
1
;
Xiaokang GONG
1
;
Xin ZHENG
1
;
Haibao WANG
1
;
Jianwei RUAN
2
Author Information
1. Orthopaedics Center, Taizhou Municipal Hospital, Taizhou 318000, Zhejiang Province, China.
2. Orthopaedics Center, Taizhou Municipal Hospital, Taizhou 318000, Zhejiang Province, China. ruan_jianwei@163.com.
- Publication Type:Journal Article
- MeSH:
3T3 Cells;
Animals;
Biocompatible Materials;
Bone Cements;
chemistry;
metabolism;
pharmacology;
Calcium Phosphates;
metabolism;
Cell Proliferation;
drug effects;
Chitosan;
chemistry;
pharmacology;
Mice;
Nanofibers;
chemistry;
Oligopeptides;
chemistry
- From:
Journal of Zhejiang University. Medical sciences
2017;46(6):593-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD). Methods: Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion. CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method. The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM). The mechanical properties were measured by universal mechanical testing instrument. The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method. Results: The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM. The infrared spectrum showed the characteristic peaks at 1633 nm and 1585 nm, indicating that RGD was successfully grafted on chitosan nanofibers. The XRD pattern showed that the bone cement had a certain curability. The stain-stress test showed that break strengths were (17.74±0.54) MPa for CPC-CSNF and (16.67±0.56) MPa for CPCP-CSNF-RGD, both were higher than that of CPC(all P<0.05). The immunofluorescence staining and MTT results indicated that MC3T3 cells grew better on CPC-CSNF-RGD after 240 min of culture(all P<0.05). Conclusion: CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
